Cd11b v450 m1 70

Manufactured by BD

CD11b-V450 (M1/70) is a lab equipment product. It is an antibody that binds to the CD11b antigen, which is expressed on the surface of certain immune cells. The core function of this product is to facilitate the identification and analysis of these CD11b-positive cells in various research and diagnostic applications.

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Lab products found in correlation

Ly 6c apc hk1, by Thermo Fisher Scientific (2 mentions) Anti f4 80 fitc bm8, by Abcam (2 mentions) Facscanto 2, by BD (2 mentions) Ccr2 pe, by R&D Systems (2 mentions) Aldehyde sulfate latex beads, by Thermo Fisher Scientific (2 mentions) Collagenase dispase, by Merck Group (2 mentions) Foxp3 pe cy7 fjk 16s, by Thermo Fisher Scientific (1 mentions) Ghost red 780 viability dye, by Cytek Biosciences (1 mentions) Ox40 pe ox 86, by BioLegend (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Cd45 bv510, by BioLegend (1 mentions) Cpg 1826, by Integrated DNA Technologies (1 mentions) Anti cd63 h5c6, by BD (1 mentions)

Spelling variants of this product

CD11b (454 citations) CD11b (M1/70, (58 citations) M1/70 (41 citations) CD11b-V450 (10 citations) V450 anti-CD11b (clone M1/70; (2 citations)

3 protocols using cd11b v450 m1 70

Isolation and Characterization of Lung Macrophages

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Mouse lung tissue was digested with collagenase-dispase (sigma-Aldrich) to generate a single cell suspension as previously described ⁵⁰. Cells were then stained with the following antibodies: Anti-F4/80-FITC (BM8, Abcam), CCR2-PE (475301, R&D System), Ly-6C-APC (HK1.4, eBiosciences), CD11b-V450 (M1/70, BD Biosciences). CD11b⁺ and Ly6C^hi cells were gated within the F4/80⁺ subpopulation, and from these, CCR2⁺ cells were sorted as previously described ⁵⁰. Characterization of exosomes markers was performed as described previously ^{8 (link), 17 (link)}. Aldehyde/sulfate latex beads (Life Technology) were coupled with 30ug of exosomes, stained with FITC-conjugated anti-CD63 (H5C6, BD Pharmingen) and analyzed by FACS. Macrophages co-incubated with OFP-mitochondria labeled MSCs were incubated with anti-F4/80⁺ and double positive F4/80⁺/RFP cells sorted by FACS. Analysis was performed using a FACS Canto II (BD Biosciences) and data analyzed using FlowJo v7.6.3 software.

Phinney D.G., Di Giuseppe M., Njah J., Sala E., Shiva S., St Croix C.M., Stolz D.B., Watkins S.C., Di Y.P., Leikauf G.D., Kolls J., Riches D.W., Deiuliis G., Kaminski N., Boregowda S.V., McKenna D.H, & Ortiz L.A. (2015). Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nature Communications, 6, 8472.

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In Vivo Immunotherapy Combination Protocol

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CpG 1826 was purchased from Integrated DNA Technologies. OX40 antibody [Anti-OX40 (CD134) antibody, rat immunoglobulin G1, OX86 clone, European Collection of Cell Cultures] was harvested and isolated from the ascites of immunodeficient mice, as previously described (33 (link)). CpG (50 µg) and/or OX40 (4 µg, 20 µg, or 100 µg) were injected IT with a 29 ½ gauge insulin syringe in 60 µL PBS every other day for three total doses (days 0, 2, 4 or days 5, 7, 9 depending on the experiment).
The following antibodies were used for flow cytometry analysis: anti-CD16/32 (93), CD45 BV510 (30-F11), CD45 FITC (30-F11), CD3 PE-Cy5 (145-2C11), CD4 BV785 (GK1.5), CD19 PE-Cy5 (6D5), CD19 APC (6D5), CD19 BV421 (6D5), Ly6G Alexa647 (1A8), IFNγ PE-Cy7 (XMG1.2), and OX40 PE (OX-86) all from BioLegend; CD8 APC-R700 (53-6.7), CD25 BB515 (PC61), NK1.1 PE-CF594 (PK136), Ly6C BV605 (AL-21), and CD11b V450 (M1/70) all from BD Biosciences; FoxP3/Transcription Factor Staining Buffer Set and FoxP3 PE-Cy7 (FJK-16s) from eBioscience. GhostRed780 Viability Dye (Tonbo Biosciences) was used for live/dead staining.

Pieper A.A., Zangl L.M., Speigelman D.V., Feils A.S., Hoefges A., Jagodinsky J.C., Felder M.A., Tsarovsky N.W., Arthur I.S., Brown R.J., Birstler J., Le T., Carlson P.M., Bates A.M., Hank J.A., Rakhmilevich A.L., Erbe A.K., Sondel P.M., Patel R.B, & Morris Z.S. (2021). Radiation Augments the Local Anti-Tumor Effect of In Situ Vaccine With CpG-Oligodeoxynucleotides and Anti-OX40 in Immunologically Cold Tumor Models. Frontiers in Immunology, 12, 763888.

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Flow Cytometry Analysis of Lung Macrophages

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Mouse lung tissue was digested with collagenase-dispase (Sigma-Aldrich) to generate a single-cell suspension as previously described50 . Cells were then stained with the following antibodies: Anti-F4/80-FITC (BM8, Abcam), CCR2-PE (475301, R&D System), Ly-6C-APC (HK1.4, eBiosciences) and CD11b-V450 (M1/70, BD Biosciences). CD11b⁺ and Ly6C^hi cells were gated within the F4/80⁺ subpopulation, and from these CCR2⁺ cells were sorted as previously described50 . Characterization of exosomes markers was performed as described previously8 (link)17 (link). Aldehyde/sulfate latex beads (Life Technology) were coupled with 30 μg of exosomes, stained with fluorescein isothiocyanate -conjugated anti-CD63 (H5C6, BD Pharmingen) and analysed using FACS. Macrophages co-incubated with Orange Fluorescent Protein (OFP)-mitochondria-labelled MSCs were incubated with anti-F4/80⁺ and double-positive F4/80⁺/RFP cells sorted using FACS. Analysis was performed using a FACS Canto II (BD Biosciences) and data analysed using the FlowJo v7.6.3 software.

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