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Urea for electrophoresis

Manufactured by Merck Group
Sourced in Italy

Urea is a chemical compound commonly used in electrophoresis, a technique used to separate and analyze biomolecules such as proteins and nucleic acids. Urea helps denature and solubilize these biomolecules, facilitating their separation and analysis. It is an essential component in various electrophoresis applications, including SDS-PAGE and DNA sequencing.

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2 protocols using urea for electrophoresis

1

Purification of Chemical Compounds

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Citric acid monohydrate (purity 99.9%, Honeywell FlukaTM, Monza, Italy), urea for electrophoresis (purity 98%, Merck Sigma-Aldrich, Milan, Italy), sodium hydroxide anhydrous (pellets, Carlo Erba, Milan, Italy), sulfuric acid (96% w/w, Carlo Erba, Milan, Italy) and water (milli-Q) were used as received without further purification. Whatman GD/X syringe filters (Fisher Scientific, Rodano, Italy) made of a porous polyvinylidene difluoride (PVDF) membrane having a pore size of 0.20 μm were used for filtration.
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2

Furan-based Chemicals Synthesis

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Nitric acid (70–71%, Aencore Chemical Co., LTD.), Urea for electrophoresis (99%, Sigma-Aldrich), i-propanol (HPLC grade, J.T. Baker), 5-hydroxymethylfurfural; 5-HMF (98%, AK scientific), 2,5-Bis (hydroxymethyl)furan; BHMF (98%, AK scientific); 2,5-dimethylfuran; 2,5-DMF (99%, Acros); 2,5-Dimethyltetrahydrofuran; DMTHF (mixture of cis- and trans-, 96%, Sigma-Aldrich); 5-Methylfurfural (5-MF, 99%, Sigma-Aldrich), 5-Methyl-2-furanmEthanol; MFA (97%, Acros), Ruthenium chloride n-Hydrate (39% metal content, UniRegion Bio-Tech), Hydrogen fluoride (HF, 48% Sigma-Aldrich), Hydrochloric acid ( ≥ 37%, Honeywell Fluka), Ethanol (99.9%, Honeywell Riedel-de-Haën), Aluminum (III) Chloride (98%, Sigma-Aldrich), Poly (ethylene glycol)-b-poly (propylene glycol)-b-poly (ethylene glycol), Pluronic P123 (Sigma-Aldrich), Furfuryl alcohol; FUL (98%, Acros Organics), Tetraethyl silicate (98%, Sigma-Aldrich), Ammonium hydroxide (28–30%, JT Baker Chemicals), Cetyltrimethylammonium bromide (98%, Alfa Aesar), Pluronic F-127(Sigma-Aldrich). Unless otherwise stated, all chemicals were used as received without further purification.
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