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Phospho and total gsk3β

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Phospho and total GSK3β is an antibody-based laboratory reagent used for the detection and quantification of glycogen synthase kinase 3 beta (GSK3β) protein, both in its phosphorylated and total forms. GSK3β is a key regulatory enzyme involved in various cellular signaling pathways.

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Lab products found in correlation

Lipopolysaccharide lps, by Merck Group (2 mentions) Recombinant human egf, by Merck Group (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (2 mentions) 3 nitrotyrosine, by Abcam (2 mentions)

Close spelling variants of this product

GSK3β (613 citations) Phospho-GSK3β (106 citations) Total GSK3β (51 citations)

2 protocols using phospho and total gsk3β

1

Comprehensive Antibody Protocol for NEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were obtained from the following sources: p65 subunit of NF-κB, phospho and total GSK3β (Cell Signaling); PCNA (Santa Cruz); 3-nitrotyrosine (Abcam), actin (Genscript), bromodeoxyuridine (BrdU, Novus Biosciences); DAPI (Invitrogen). TUNEL (Roche Applied Science) was performed according to the manufacturer’s instructions as in 22 (link). Appropriate secondary antibodies for immunohistochemistry and SDS-PAGE were obtained from Molecular Probes and Jackson ImmunoResearch Laboratories. Cetuximab (Dose: 100 μg/day i.p. for 3 days prior to experiments) was a generous gift of Jennifer Grandis (University of Pittsburgh, Pittsburgh, PA). Human Recombinant EGF was from EMD Millipore (400 ng/mL media in vitro, 0.5ng per μL of NEC formula in vivo). Lithium chloride (LiCl) from J.T. Baker (100μM). Lipopolysaccharide (LPS) (Escherichia coli 0111:B4 purified by gel-filtration chromatography, >99% pure) was obtained from Sigma-Aldrich and concentrations used were those that we have demonstrated to be present in mice and humans with NEC (50 μg/mL for cells, 5mg/kg for all mice3 with the exception of the TLR4ΔIEC-OVER mice who are sensitive to LPS and required a decreased dosage (2.5 mg/kg).
Good M., Sodhi C.P., Egan C.E., Afrazi A., Jia H., Yamaguchi Y., Lu P., Branca M.F., Ma C., Prindle T J.r., Mielo S., Pompa A., Hodzic Z., Ozolek J.A, & Hackam D.J. (2015). Breast milk protects against the development of necrotizing enterocolitis through inhibition of Toll Like Receptor 4 in the intestinal epithelium via activation of the epidermal growth factor receptor. Mucosal immunology, 8(5), 1166-1179.
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2

Comprehensive Antibody Protocol for NEC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were obtained from the following sources: p65 subunit of NF-κB, phospho and total GSK3β (Cell Signaling); PCNA (Santa Cruz); 3-nitrotyrosine (Abcam), actin (Genscript), bromodeoxyuridine (BrdU, Novus Biosciences); DAPI (Invitrogen). TUNEL (Roche Applied Science) was performed according to the manufacturer’s instructions as in 22 (link). Appropriate secondary antibodies for immunohistochemistry and SDS-PAGE were obtained from Molecular Probes and Jackson ImmunoResearch Laboratories. Cetuximab (Dose: 100 μg/day i.p. for 3 days prior to experiments) was a generous gift of Jennifer Grandis (University of Pittsburgh, Pittsburgh, PA). Human Recombinant EGF was from EMD Millipore (400 ng/mL media in vitro, 0.5ng per μL of NEC formula in vivo). Lithium chloride (LiCl) from J.T. Baker (100μM). Lipopolysaccharide (LPS) (Escherichia coli 0111:B4 purified by gel-filtration chromatography, >99% pure) was obtained from Sigma-Aldrich and concentrations used were those that we have demonstrated to be present in mice and humans with NEC (50 μg/mL for cells, 5mg/kg for all mice3 with the exception of the TLR4ΔIEC-OVER mice who are sensitive to LPS and required a decreased dosage (2.5 mg/kg).
Good M., Sodhi C.P., Egan C.E., Afrazi A., Jia H., Yamaguchi Y., Lu P., Branca M.F., Ma C., Prindle T J.r., Mielo S., Pompa A., Hodzic Z., Ozolek J.A, & Hackam D.J. (2015). Breast milk protects against the development of necrotizing enterocolitis through inhibition of Toll Like Receptor 4 in the intestinal epithelium via activation of the epidermal growth factor receptor. Mucosal immunology, 8(5), 1166-1179.
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