Two-week-old SD rats (Vital River Laboratory Animal Technology, China) were used to extract rat bone mesenchymal stem cells (BMSCs). The medullary cavity of rat's femur and tibia were rinsed with MEM-α medium (Gibco, USA) containing 10% fetal bovine serum (Gibco, USA). BMSCs were cultured in standard conditions (37 °C, 95%/5% air/CO2) and seeded in complete media, which consisted of MEM-α containing 10% fetal bovine serum and 1% penicillin and streptomycin (Macklin, China). The culture medium was changed every 2 days. Cells were digested with 0.05% trypsin-ethylenediaminetetraacetic acid (EDTA) (Gibco, USA) for following experiments.
Penicillin and streptomycin
Manufactured by Maclin Biochemical Technology
Sourced in China
Penicillin and streptomycin are two commonly used antibiotics. Penicillin is a beta-lactam antibiotic that inhibits the synthesis of the bacterial cell wall, while streptomycin is an aminoglycoside antibiotic that interferes with protein synthesis. These antibiotics are used to treat a variety of bacterial infections.
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Lab products found in correlation
2 protocols using penicillin and streptomycin
1
Rat Bone Mesenchymal Stem Cell Extraction
2
Hep-2 Cells for Laryngeal Cancer
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Human laryngeal cancer cell line Hep‐2 purchased from Qi Biotechnology Co.; fetal bovine serum (FBS) purchased from ExCell Co.; CuE purchased from Macklin Co.; RPMI1640 medium purchased from Hyclone Co.; CD133 antibody purchased from Immunoway Co.; ABCG2 rabbit anti‐human monoclonal antibody purchased from Immunoway Co.; P‐gp mouse anti‐human monoclonal antibody purchased from Immunoway Co.; Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) purchased from Wuhan Seville Biotechnology Co., Ltd.; sheep anti‐mouse secondary antibody purchased from Wuhan Seville Biotechnology Co., Ltd.; penicillin and streptomycin purchased from Macklin Co.; basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), and B27 purchased from Beijing BoaoSen Biotechnology Co., Ltd.; c‐MYC antibody purchased from Wuhan Seville Biotechnology Co., Ltd.; 20 nude mice, mass (15 ± 2) g, were purchased from Hunan Sja laboratory Animal Co., Ltd, license No. SCXK (Xiang) 2019‐0004, SPF grade, housed in four cages, five rats/cage, at the Experimental Animal Center of Lanzhou University. All animal studies and experimental procedures were approved by the Animal Care and Use Committee of the animal facility at Lanzhou University.
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