2 2 diphenyl 1 picrylhydrazyl (dpph)

The chemicals used in the present work were purchased from various companies. Sodium nitrite, potassium persulfate, dipotassium hydrogenphosphate, ferric chloride, monosodium dihydrogenphosphate, potassium ferricyanide, trichloroacetic acid, disodium hydrogenphosphate, Rutin, Mueller-Hinton agar (MHA), and Folin-Ciocalteu reagent were purchased from Merck (Darmstadt, Germany), gallic acid from Riedel-de-Haen (Seelze, Germany), ammonium molybdate, linoleic acid, ferrous chloride tetrahydrate and aluminum chloride from BDH Labs (Cambridge, England), DPPH (2,2-diphenyl-1-picrylhydrazyl) and ascorbic acid from MP Biomedicals (Illkirch, France), sodium acetate from Daejung (Siheung City, Korea), ammonium thiocyanate from Alfa-Aesar (Karlsruhe, Germany), amoxicillin from GlaxoSmithKline (Karachi, Pakistan) and trichloroacetic acid, TPTZ (2,4,6 tripyridyl-s-triazine), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were purchased from Sigma-Aldrich (Steinheim, Germany).

2,2-Diphenyl-1-picrylhydrazyl (DPPH) (Sigma Aldrich, Germany) and ((s)-(-)-6 hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid) (Sigma Aldrich, Denmark). n-hexane (Frutarom Ltd, Israel). Methanol (Lobachemie, India), The solvents used for the extraction of plant material were of HPLC grade. Sigma-Aldrich (USA) provided porcine pancreatic lipase, starch, acarbose, DPPH (2,2-diphenyl-1-picrylhydrazyl), PNPP (p-nitrophenyl palmitate), and orlistat, while MP Biochemicals (Illkirch, France) provided porcine pancreatic α-amylase. All the chemicals used in the experiments were of analytical grade. O. basilicum seeds were obtained from different regions in Palestine. The plant seeds were characterized in the Pharmacy Department at An-Najah National University. The plant material was collected in accordance with the WHO Guidelines for the Assessment of Herbal Medicines and Legislation.
The rotary evaporator (Heidolph OB2000, VV2000, Germany), the grinder (Moulinex model, Uno, China), the balance (Radwag, AS 220/c/2, Poland), and the filter papers (Machrery-Nagel, MN 617, Whatman no. 1, USA) were all used.