Sources of commercial antibodies were as follows: anti-FUS (Santa Cruz, Dallas, TX, USA; 4H11), anti-Pur-alpha (Abcam, Cambridge, UK; ab77734), anti-Pur-alpha (Abcam; ab79936), anti-HA (Santa Cruz; Y-11), anti-HA (Santa Cruz; F-7), anti-Flag (Sigma-Aldrich, St. Louis, MO, USA; M2 and M2 affinity gel), anti-Flag fluorescein isothiocyanate (FITC) conjugated (Sigma-Aldrich; M2), anti-Phospho-eIF2-alpha (Cell Signaling Technology, Beverly, MA, USA; D9G8), anti-eIF2-alpha (Cell Signaling Technology; D7D3), anti-cleaved caspase-3 (Cell Signaling Technology; Asp175), anti-NeuN (Merck Millipore, Billerica, MA, USA; A60), anti-TIAR (BD Biosciences, Erembodegem, Belgium), anti-GAPDH (Chemicon-Merck Millipore), anti-puromycin 3RH11 monoclonal antibody (Kerafast, Boston, MA, USA), anti-FUS (Abcam; ab84078), anti-Islet-1/2 (DSHB, Iowa City, IA, USA; 39.4D5). Mouse monoclonal antibody specific for ribosomal protein S19 were prepared in our laboratory.31 (link) FITC, Rhodamine, and aminomethylcoumarin-conjugated affinity-purified secondary anti'bodies, selected for absent cross-reaction, were from Jackson ImmunoResearch (West Grove, PA, USA).
Di Salvio M., Piccinni V., Gerbino V., Mantoni F., Camerini S., Lenzi J., Rosa A., Chellini L., Loreni F., Carrì M.T., Bozzoni I., Cozzolino M, & Cestra G. (2015). Pur-alpha functionally interacts with FUS carrying ALS-associated mutations. Cell Death & Disease, 6(10), e1943-.
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