1640 culture medium (Gibco), Opti-MEM (Gibco), FBS (Gibco), penicillin, streptomycin, trypsin, RIPA, and Cell Counting Kit-8 (Gibco), rabbit anti-rat HIF-1α (Novus Biologicals), mouse anti-rat VEGF (Novus Biologicals), mouse anti-rat glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Abcam), DNA Ladder (Solarbio, China), SonoVue (Bracco, Italy), HIF-1α shRNA were synthesized by the Genomics Institute, Sonitron 2000V (Nepa Gene, Japan), Microscope (Nikon, Japan), High Speed Freezing Microcentrifuge (SCILOGEX, Americ ), and Multiskan GO (Thermo Scientific, America).
Dna ladder
Manufactured by Solarbio
Sourced in China
A DNA ladder is a molecular weight standard used to determine the size of DNA fragments in gel electrophoresis. It consists of a mixture of DNA fragments of known sizes, which are used as a reference to estimate the size of unknown DNA samples.
Automatically generated - may contain errors
Lab products found in correlation
Multiskan go, by Thermo Fisher Scientific (2 mentions)
1640 culture medium, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 (cck8), by Thermo Fisher Scientific (1 mentions)
Sonitron 2000v, by Nepa Gene (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Sonovue, by Bracco (1 mentions)
Rpmi 1640 medium, by Solarbio (1 mentions)
Sulfosuccinimidyl 4 n maleimidomethyl cyclohexane 1 carboxylate sulfo smcc, by Merck Group (1 mentions)
Safranin o fast green, by Wuhan Servicebio Technology (1 mentions)
Agarose powder, by Biowest (1 mentions)
Hematoxylin and eosin h e, by Wuhan Servicebio Technology (1 mentions)
Enzyme linked immunosorbent assay elisa kits, by MultiSciences Biotech (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Complete freund s adjuvant cfa, by Chondrex (1 mentions)
Caspase 3 activity assay kit, by Beyotime (1 mentions)
E coli bl21 de3, by Transgene (1 mentions)
Protein markers, by Solarbio (1 mentions)
E coli trans t1, by Transgene (1 mentions)
Hrv 3c protease, by Takara Bio (1 mentions)
3 protocols using dna ladder
1
Hypoxia Regulation of Angiogenesis
2
Synthesis and Characterization of Compounds for Rheumatoid Arthritis Study
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Oligonucleotides and peptide were synthesized by Sangon Biotech (Shanghai, China) and Huajin Biotechnology (Shanghai, China). Agarose powder was obtained from Biowest (Nuaillé, France). Sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (sulfo-SMCC) was supplied by Sigma (Darmstadt, Germany). EN was purchased from Shanghai CP Guojian Pharmaceutical (Shanghai, China). Cell counting kit 8 (CCK8), annexin V-FITC apoptosis staining/detection kit and caspase 3 activity assay kit were purchased from Beyotime Biotechnology (Shanghai, China). RPMI Medium 1640 was obtained from Beijing Solarbio Science & Technology (Beijing, China). Fetal bovine serum (FBS) was provided by BI (Kibbutz, Israel). Complete Freund's adjuvant (CFA) and immune grade chick type II Collagen were purchased from Chondrex (Woodinville, USA). Freund's adjuvant incomplete (IFA) was purchased from Sigma (Saint Louis, USA). Enzyme linked immunosorbent assay (ELISA) kits were purchased from MultiSciences Biotech (Hangzhou, China). DNA Ladder and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Beijing Solarbio Science & Technology (Beijing, China). Hematoxylin and eosin (H&E) and Safranin O-fast green were provided from Servicebio (Wuhan, China). All chemicals and materials were used as received without any further modifications.
3
Recombinant Protein Expression in E. coli
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E. coli TransT1 and E. coli BL21 (DE3) were obtained from TransGen (Beijing, China). DNA polymerase, DNA ladder, and protein markers were from Solarbio (Beijing, China). Exonuclease, HRV 3C Protease, and DNA ligase were from Takara (Dalian, China). Boc–Gln–Ala–Arg–AMC was from Bachem (Bubendorf, Switzerland). 6–isopropyl–β–d–thiogalactopyranoside (IPTG) was from Sigma–Aldrich (St. Louis, MO, USA). His antibody was from Beyotime (Shanghai, China). All other chemicals were of the highest reagent grade commercially available.
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