HaCaT was seeded in 24-well plates at 19 × 103 cells/well up to 80% confluence and then further cultured in fresh DMEM containing ME, ANDRO, NEO, 14DAP, or 14DAP11-12, with or without TNF-α (10 ng/mL, as cytokine generator) for 24 h. Supernatants were collected and used in the analysis of newly secreted interleukins. IL-8 was quantified using a sandwich ELISA assay kit (Peprotech, Rock Hill, NJ, USA), according to the manufacturer’s protocol. The assay was performed in 2 replicates of three independent experiments (n = 3).
Sandwich elisa assay kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Sandwich ELISA assay kit is a laboratory equipment designed for the detection and quantification of target analytes in samples. It utilizes a solid-phase enzyme immunoassay technique to capture and measure the concentration of specific proteins or other biomolecules. The kit includes the necessary components, including microplate, antibodies, and detection reagents, to perform the assay.
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Lab products found in correlation
2 protocols using sandwich elisa assay kit
1
Interleukin-8 Secretion in HaCaT Cells
2
Interleukin Secretion in HDFa Cells
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HDFa were seeded in 24-well plates at 1 × 104 cells/well up to 80% confluence and then further cultured in fresh DMEM containing ME, ANDRO, NEO, 14DAP or 14DAP11-12, with or without TNF-α (10 ng/mL; as cytokines generator) for 24 h. Supernatants were collected and used in analysis of newly secreted interleukins. IL-6 and IL-8 were quantified using a sandwich ELISA assay kit (Peprotech, Rock Hill, NJ, USA), according to the manufacturer’s protocol. The assay performed in 2 replicates of three independent experiments (n = 3).
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