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Arnt sirna

Manufactured by Thermo Fisher Scientific
Sourced in United States

ARNT siRNA is a laboratory reagent used for RNA interference (RNAi) experiments. It is designed to target and silence the expression of the ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator) gene in cell-based assays. ARNT siRNA can be used as a research tool to investigate the role of the ARNT protein in various biological processes.

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3 protocols using arnt sirna

1

Knockdown of ARNT in SIM2 Cells

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ARNT siRNA purchased from Ambion (Austin, TX, USA) was introduced to SIM2‐transfectants using Thermo Scientific DharmaFECT Transfection Reagents (Thermo Fisher Scientific, Waltham, MA, USA). Quantitative real‐time PCR and flow cytometry were carried out after siRNA treatment of the SIM2‐transfectants.
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2

Targeted Gene Silencing in Cells

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AHR siRNA (siRNA ID number: s1199), ARNT siRNA (siRNA ID number: s1613) and MMP1 siRNA (siRNA ID number: s8847) were purchased from Ambion’s Silencer Select pre-designed siRNA library (Grand Island, NY). A non-specific, negative control siRNA (negative control #1, Ambion) was used as the control. Cells were grown to 70–80% confluence in 6-well plates and treated with the siRNAs mixed with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) in OptiMEM I (Invitrogen, Carlsbad, CA) at a final concentration of 50 nM for 24 hours, according to the manufacturers’ instructions. Cells were then treated with or without TGFβ and/or FICZ as described.
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3

Reverse Transfection for Gene Silencing

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Gene silencing was achieved by a reverse transfection procedure using Lipofectamine® RNAiMAX (Life technologies) in accordance with the manufacturer’s guidelines. Therefore, cells were seeded on 24-well plates and mixed with the transfection mixture containing either ARNT siRNA (#s1613, Ambion) or BLOCK-iT™ Fluorescent Control siRNA (#442926, Life technologies). Subsequently cells were incubated overnight and subjected to irradiation. To gain protein lysates for Western blot analysis, the transfection procedure was performed in 6-well plates.
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