The anti-P2X7R antibody was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-p38, anti-p-p38, anti-IL-1β, anti-IL-6, anti-TNF-a, anti-IL-10 and anti-Synaptotagmin 1 antibodies were obtained from Affinity Biosciences (Cincinnati, OH, USA). The anti-Iba1 antibody was obtained from Wako (Wako Pure Chemical Industries, Ltd). An anti-GAPDH was obtained from Bioworld. The anti-BDNF antibody and the P2X7R-specific agonist BzATP were obtained from Abcam (Cambridge Science Park, Cambridge, UK). An FD Rapid GolgiStain Kit was obtained from FD Neurotechnologies, Inc. (Guilford, MD, USA).
Anti p2x7r antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The Anti-P2X7R antibody is a laboratory reagent used to detect and study the P2X7 receptor, a member of the P2X family of ATP-gated ion channels. The antibody can be used in various experimental techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to identify and localize the P2X7 receptor in biological samples.
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Lab products found in correlation
Anti bdnf antibody, by Abcam (1 mentions)
Anti il 6, by Affinity Biosciences (1 mentions)
Anti il 1β, by Affinity Biosciences (1 mentions)
Anti gapdh, by Bioworld Technology (1 mentions)
Anti p p38, by Affinity Biosciences (1 mentions)
Anti il 10, by Affinity Biosciences (1 mentions)
Anti p 38, by Affinity Biosciences (1 mentions)
Anti iba1 antibody, by Fujifilm (1 mentions)
Hoechst, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 555, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti p2x7r antibody
1
Neuroinflammatory Signaling Pathway Analysis
2
Immunofluorescent Localization of P2X7 Receptor
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Osteoclast-like cell-enriched populations seeded in coverslips were rinsed with PBS, fixed in ice-cold methanol, and permeabilized with PBS-0.1% Triton X-100. Then, the covers were rinsed with PBS and blocked with PBS-1% BSA for 1 h at room temperature. After that, cells were washed with PBS and incubated with an anti-P2X7R antibody (Santa Cruz Biotechnologies, TX, USA), at 4°C overnight. Then, cells were washed with PBS and incubated for 1 h with the secondary antibody Alexa Fluor-555 (Thermo Fisher Scientific, Waltham, MA, USA). For nuclei staining, cells were incubated with Hoechst (Thermo Fisher Scientific, Waltham, MA, USA) for 10 min. Samples were finally washed with PBS, mounted in Dako anti-fading reactive (Dako, Glostrup, Denmark) and stored at 4 °C until use.
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