The binding kinetics of ABY-027 and ABY-271 to HSA and MSA were determined using Surface Plasmon Resonance (Biacore 8K, Cytiva). In these experiments, HSA or MSA were immobilized to the chip (Series S Sensor Chip CM5, Cytiva) using amine coupling kit type 2 (Cytiva).
Amine coupling kit type 2
Manufactured by Cytiva
The Amine Coupling Kit Type 2 is a laboratory tool designed for the covalent immobilization of amine-containing molecules onto a solid support. It provides a simple and efficient method for the attachment of proteins, peptides, or other amine-bearing compounds to a variety of surfaces. The kit includes all the necessary reagents and buffers to facilitate the coupling reaction.
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Lab products found in correlation
2 protocols using amine coupling kit type 2
1
Binding Kinetics of ABY-027 and ABY-271 to HSA and MSA
2
Binding Kinetics of ABY-027 and ABY-271 to HER2
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The binding kinetics of ABY-027 and ABY-271 to HER2, human serum albumin (HSA) and mouse serum albumin (MSA) were determined using Surface Plasmon Resonance (Biacore 8K, Cytiva, Uppsala, Sweden). Binding to HER2 by either ABY-027 or ABY-271 (10 nM) was performed in buffer or in the presence of HSA or MSA (100 nM). In these experiments, HER2 was immobilized to the chip (Series S Sensor Chip CM5, Cytiva) using amine coupling kit type 2 (Cytiva). Then, 10 mM sodium acetate pH 4.5 was used as an immobilization buffer.
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