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2 protocols using thiophanate methyl

1

Pesticide and Heavy Metal Analysis Protocol

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All pesticides standards including α-BHC(100 mg L−1), β-BHC (100 mg L−1), γ-BHC (100 mg L−1), δ-BHC (100 mg L−1), p,p′-DDE (100 mg L−1), o,p′-DDT (100 mg L−1), p,p′-DDT (100 mg L−1), p,p′-DDD (100 mg L−1), azoxystrobin (99.0%), carbendazim (99.0%), chlorothalonil (98.9%), chlorpyrifos (99.5%), cypermethrin (98.2%), chlorantraniliprole (98.2%), dichlorvos (99.7%), difenoconazole (99.2%), fenpropathrin (98.9%), isocarbophos (98.9%), procymidone (99.5%), prochloraz (99.2%), pyraclostrobin (98.1%), thiophanate-methyl (99.3%), tebuconazole (99.5%) and λ-cyhalothrin (93.5%) were purchased from Dr Ehrenstorfer GmbH (Augsburg, Germany). Acetonitrile (HPLC grade) were provided by Bangyi Chemical Co., Ltd. (Hangzhou, China). Anhydrous magnesium sulphate, anhydrous sodium sulphate and n-hexane were of analytical grade. Primary secondary amine (PSA) was provided by Agela Technologies.
Each single element stock solution (1000 mg L−1) of Cu, Zn, Cr, Ni, Pb, As, Cd, and Hg were purchased from National Standard Material Research Centre (Beijing, China). Guaranteed reagent nitric acid (HNO3) was provided by Fanhong Trading Co., Ltd. (Guangzhou, China).
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2

Analytical Standards for Pesticide Detection

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Analytical standards of flubendiamide (97%), triadimefon (99%), tebuconazole (99%), difenoconazole (98%), carbendazim (98%), fenaminosulf (99%), and thiophanate-methyl (99%) were purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany), and analytical standards of triazophos, isoprothiolane, hexaconazole, propiconazole, prochloraz, tricyclazole, abamectin, buprofezin, emamectin benzoate, chlorantraniliprole, pymetrozine, imidacloprid, nitenpyram, thiamethoxam, acetamiprid, iprodione, procymidone, and diniconazole (all at 100 μg/ml) were bought from the Agro-Environmental Protection Institute (Tianjin, China). Methanol (MeOH; LC grade) and acetonitrile (ACN; LC grade) were obtained from Thermo Fisher Scientific, Inc. (Fairlawn, USA). Ammonium acetate (HPLC grade) and formic acid (HPLC grade) were provided by Tedia Company, Inc. (Fairfield, USA). Primary-secondary amine (PSA, 40–63 µm) and octadecyl silane (C18, 50 µm) sorbents were purchased from Shanghai Anpel Scientific Instrument Co., Ltd. (Shanghai, China). Purified water was prepared by a Milli-Q reagent water system (Millipore, Milford, MA, USA).
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