Tetrazolium dye 3 4 5 di methylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt

PLGA (50:50 DLG 5E) was purchased from Lakeshore Biomaterials (Birmingham, AL, USA). Clindamycin HCL was a generous gift from Samjin Pharmaceutical Company. PEI (M_W 1.8 kDa), poly(vinyl alcohol) (PVA), 2,2,2-tribromoethanol and tert-amyl alcohol (2-methyl-2-butanol) (Avertin anesthesia component), Mayer’s hematoxylin, eosin-Y disodium salt, Nile red, tetrazolium dye 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bacto^TM tryptic soy broth (TSB) medium was purchased from BD Biosciences (Sparks, MD, USA). The LIVE/DEAD^® Baclight^TM bacterial viability kit (Molecular Probes) was purchased from Life Technologies (Eugene, OR, USA). The Roswell Park Memorial Institute (RPMI) 1640 medium, trypsin, fetal bovine serum (FBS), and penicillin-streptomycin were purchased from Hyclone, Thermo Fisher Scientific Inc. (Waltham, MA, USA). Phosphate buffered saline (PBS; 20×) was purchased from Biosesang (Seoul, Korea). All other reagents and solvents used were of the highest analytical grade.

The dimeric dipeptides GK-6 and GK-2 were synthesized on the base of murine NGF at the Zakusov Institute of Pharmacology (Moscow, Russia).
Inhibitors of PI3K (LY294002) and MAPK (PD98059) were purchased from Tocris Bioscience (Bristol, UK). The tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT),was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s Modified Eagle’s medium was purchased from HyClone Laboratories (Logan, UT, USA). Fetal bovine serum was obtained from Gibco (Langley, OK, USA). Glutamine was purchased from ICN Pharmaceuticals. Inc. (Costa Mesa, CA, USA). Poly-D-lysine was purchased from BD Biosciences (San Jose, CA, USA). DC protein assay was purchased from Biorad (Hercules, CA, USA). Anti-TrkA, anti-pTrkA, anti-AKT1/2/3, anti-pAKT1/2/3, anti-ERK1/2, anti-pERK1/2 antibodies and enhanced chemiluminescence kits were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-β-actin antibodies and horseradish peroxidase conjugated antibodies were purchased from Abcam (Cambridge, MA, USA).