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Cy 3 labelled control mirna

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Cy-3-labelled control miRNA is a synthetic miRNA molecule labelled with the fluorescent dye Cy-3. It is used as a control sample in various miRNA detection and analysis techniques, such as qRT-PCR and microarray experiments.

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Lab products found in correlation

Opti mem 1, by Thermo Fisher Scientific (2 mentions) Siport neofx transfection reagent, by Thermo Fisher Scientific (2 mentions) Mirna 132, by Thermo Fisher Scientific (2 mentions) αmirna 132, by Thermo Fisher Scientific (2 mentions) Label it mirna labeling kit, by Mirus Bio (2 mentions) Control mirna, by Thermo Fisher Scientific (2 mentions)

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Control miRNA (26 citations)

2 protocols using cy 3 labelled control mirna

1

miRNA-132 Transfection in Cells

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The Cy-3-labelled Control miRNA was purchased from Life Technologies. miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies) were labelled using Label IT miRNA Labeling Kit (Mirus) according to manufacturer’s protocol. Cells were transfected with Control miRNA (Life Technologies), miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies). The miRNAs were transfected with siPORT NeoFX transfection reagent (Life Technologies) at a concentration of 50 nM and 1 × Opti-MEM I (Life Technologies). All transfections were completed according to the manufacturer’s protocols for 24 h.
Connor Y., Tekleab S., Nandakumar S., Walls C., Tekleab Y., Husain A., Gadish O., Sabbisetti V., Kaushik S., Sehrawat S., Kulkarni A., Dvorak H., Zetter B., R. Edelman E, & Sengupta S. (2015). Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. Nature Communications, 6, 8671.
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2

miRNA-132 Transfection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Cy-3-labelled Control miRNA was purchased from Life Technologies. miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies) were labelled using Label IT miRNA Labeling Kit (Mirus) according to manufacturer's protocol. Cells were transfected with Control miRNA (Life Technologies), miRNA-132 (Life Technologies) and αmiRNA-132 (Life Technologies). The miRNAs were transfected with siPORT NeoFX transfection reagent (Life Technologies) at a concentration of 50 nM and 1 × Opti-MEM I (Life Technologies). All transfections were completed according to the manufacturer's protocols for 24 h.
Connor Y., Tekleab S., Nandakumar S., Walls C., Tekleab Y., Husain A., Gadish O., Sabbisetti V., Kaushik S., Sehrawat S., Kulkarni A., Dvorak H., Zetter B., R. Edelman E, & Sengupta S. (2015). Physical nanoscale conduit-mediated communication between tumour cells and the endothelium modulates endothelial phenotype. Nature Communications, 6, 8671.
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