Western blot analysis was performed to determine the protein expression of BMPR2, SOX2 and GAPDH. Total protein from the cells were lysed by RIPA buffer. GAPDH was used as an endogenous normalizer. Polyclonal rabbit anti-human BMPR2 (1:1000, sc-20737; Santa Cruz Biotechnology, Santa Cruz, CA, USA), SOX2 (1:1000; sc-17319; Santa Cruz Biotechnology) and GAPDH (1:1000; sc-367714, Santa Cruz Biotechnology) antibodies were used.
Sc 367714
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-367714 is a research-use-only laboratory equipment product from Santa Cruz Biotechnology. It is designed for conducting various scientific experiments and analyses. The core function of this product is to facilitate the research and exploration of biological samples and materials.
Automatically generated - may contain errors
2 protocols using sc 367714
1
Protein Expression Analysis of BMPR2, SOX2
2
Multiplex Antibody Profiling and Gene Silencing
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Rabbit polyclonal antibody against GAPDH (sc-367714) human cyclin D1 (sc-718) or smooth muscle myosin heavy chain 11 (ab53219) and mouse monocloal antibody against smooth muscle actin (sc-53142) or BrdU (sc-32323) were all purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Rabbit polyclonal antibody against human NOR1 was purchased from Abcam (ab188752; Cambridge, MA, USA). Lipofactimine 200, TaqMan gene expression assays, and Trizol were purchased from Invitrogen Biotechnology, Inc. (Waltham, MA, USA). The specific siRNA targeted for human NOR1 gene (NOR1-siRNA) and negative-control siRNA (NC-siRNA) were synthesized by Shanghai Bio-Engineering Co. Ltd (Shanghai, People’s Republic of China).
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