Heptaldehyde

Manufactured by Merck Group

Sourced in China, United States

Heptaldehyde is an organic compound with the chemical formula C7H14O. It is a clear, colorless liquid with a pungent odor. Heptaldehyde is commonly used as an intermediate in the synthesis of other chemicals and as a solvent in various industrial applications.

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2 protocols using heptaldehyde

Analyzing AOX Activity in Silkworm Extracts

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AOX activity in the PG, antenna, and ovary extracts of the female silkworm moths sampled on day 1 without mating were measured as described by Rybezynski et al. (1989) (link). Total proteins (40 μg) in each lane were separated using 8% native-PAGE, and the gels were subsequently stained for AOX activity using 5 mmol/liter AOX substrate, such as benzaldehyde (Sigma), heptaldehyde (Sigma), salicylaldehyde (J&K Chemical Ltd. Shanghai, China), propionaldehyde (J&K), and vanillic aldehyde (J&K), with 0.4 mmol/liter 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide (MTT) and 0.1 mmol/liter phenazine methosulfate (PMS) as the electron acceptors. Upon AOX reaction, the reducing agent precipitated with a purple color, making the blue band visible in the native gel (Marelja et al. 2014 (link)).

Zhang Y., Yang Y., Shen G., Mao X., Jiao M, & Lin Y. (2020). Identification and Characterization of Aldehyde Oxidase 5 in the Pheromone Gland of the Silkworm (Lepidoptera: Bombycidae). Journal of Insect Science, 20(6), 31.

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Synthesis and Characterization of Isotopically Labeled Compounds

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γ-Nonalactone (> 97%), isopropyl bromide, oxalyl chloride (> 98%), diisobutylaluminium hydride (1.0 M in toluene), deuterium chloride (35% in ²H₂O), sodium borohydride (98%), heptaldehyde (95%), selenium dioxide, deuterium gas, and hydrobromic acid (48%, aq.) were purchased from Sigma-Aldrich (MO, USA). Benzoic acid (AR), triphenylphosphine (97%), and Pd on activated carbon (10%) were purchased from AK Scientific (CA, USA). ¹³C₂-bromoacetic acid, deuterium oxide, and deuterochloroform were purchased from Cambridge Isotope Laboratories (MA, USA). Magnesium sulfate, sodium hydroxide, potassium hydroxide, tartaric acid, and all organic solvents were purchased from ECP Ltd (Auckland, New Zealand). A Sartorius (Göttingen, Germany) Arium Pro Ultrapure Water System was used for Type 1 water production. Chemicals were used as purchased, unless otherwise specified.

Miller G.C., Barker D., Pilkington L.I, & Deed R.C. (2023). Synthesis of a novel isotopically labelled standard for quantification of γ-nonalactone in New Zealand Pinot noir via SIDA-SPE-GC–MS. Analytical and Bioanalytical Chemistry, 415(20), 5035-5047.

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