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S100a8 a9 elisa kit

Manufactured by R&D Systems
Sourced in United States

The S100a8/a9 Elisa kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the levels of S100a8/a9 (also known as Calprotectin) in biological samples.

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2 protocols using s100a8 a9 elisa kit

1

ELISA Validation of Lung Proteins

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Lung of six mice were used for Elisa validation. Three mice in control group and the other three in infection group. The MMP-9 Elisa kit (R&D, MMPT90, the detection range was 0.08∼5 pg/mL) and S100a8/a9 Elisa kit (R&D, DY8596-05, the detection range was 125∼5,000 pg/mL) were used according to the manufacturer’s instructions to determine the concentration of proteins in the tissue homogenate. The proinflammatory factor IL-6 (R&D, DY406-05, the detection range was 15.6∼1,000 pg/mL) was also determined to show the host inflammatory response.
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2

Plasma DAMP Quantification by ELISA

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The plasma DAMPs were assessed by commercially available ELISA kits, according to the manufacturer's instructions, as follows: the S100A8/A9 ELISA kit (DS8900) from R&D Systems (Minneapolis, MN, USA), the HMGB1 ELISA kit (NBP2-62766) and SP-A ELSA kit (NBP2-76692) from Novus Biologicals (Centennial, CO, USA), the CIRBP ELISA kit (CY-8103) from MBL Life Sciences (Nagoya, Japan), and the Histone H4 ELISA kit (Ab156909) from Abcam (Cambridge, UK).
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