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Calcofluor white stain cfw

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Calcofluor White stain (CFW) is a fluorescent dye commonly used in microscopy and microbiology. It binds to cellulose and chitin, which are structural components found in the cell walls of many fungi and some bacteria. The dye emits a bright blue fluorescence when exposed to ultraviolet or blue light, allowing for the visualization and identification of these microorganisms.

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Lab products found in correlation

Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions) Fm1 43, by Thermo Fisher Scientific (1 mentions) Cyanine5 carboxylic acid cy5, by Lumiprobe (1 mentions)

Spelling variants of this product

Calcofluor white (369 citations) Calcofluor White Stain (74 citations) Calcofluor white (CFW) (63 citations) Calcofluor (51 citations)

2 protocols using calcofluor white stain cfw

1

Fluorescent Dye Labeling Protocol

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All chemicals were purchased from commercial sources as reagent grade and used without further purification unless stated otherwise. Fluorescent dyes were obtained in their carboxylic acid form, as N-hydroxysuccinimid-esters or isothiocyanates for coupling and were used without further purification: Fluorescein isothiocyanate (FITC) (Sigma Aldrich, Vienna, Austria)) 6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid (NBD) (Sigma Aldrich, Vienna, Austria), 5(6)-carboxy-2′,7′-dichlor-fluorescein-D (DFFDA) (Sigma Aldrich, Vienna, Austria), Calcofluor White stain (CFW) (Sigma Aldrich, Vienna, Austria), 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY 630/650), (Lumiprobe GmbH, Hannover, Germany), Cyanine 5-carboxylic acid (Cy5) (Lumiprobe GmbH, Hannover, Germany), 3-Carboxy-6,8-difluoro-7-hydroxycoumaryl succinimidyl ester (Ocean Blue); 5-Carboxytetramethylrhodamine-NHS (TAMRA) (Bio-Techne Ltd, Abingdon, UK), 10-(5-amino-2-methylphenyl) silicon-rhodamine (SiR) (prepared in house19 (link)), (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino)Styryl)pyridinium dibromide) (FM1-43) (ThermoFischer Scientific, Watham, MA, USA).
Pfister J., Lichius A., Summer D., Haas H., Kanagasundaram T., Kopka K, & Decristoforo C. (2020). Live-cell imaging with Aspergillus fumigatus-specific fluorescent siderophore conjugates. Scientific Reports, 10, 15519.
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2

Fungal Biofilm Imaging Protocol

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Biofilms for imaging were cultured in MatTek dishes (MatTek #P35G-1.0-14-C) by seeding 105 spores/mL of GMM with 2 mL per dish for 24 hours at 37°C with 5% CO2 at 21% O2 or 0.2% O2. Calcofluor white stain (CFW) (Sigma) was used to visualize the hyphae at a final concentration of 25 μg/mL for 15 minutes.
Kowalski C.H., Kerkaert J.D., Liu K.W., Bond M.C., Hartmann R., Nadell C.D., Stajich J.E, & Cramer R.A. (2019). Fungal Biofilm Morphology Impacts Hypoxia Fitness and Disease Progression. Nature microbiology, 4(12), 2430-2441.
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