Miniprep kit

Plasmid pUC57-ORF5 was used as a template for amplification using the N34-F/R primer set. The amplified target fragment was cloned into the pMD-19T Simple vector (TaKaRa, Dalian, China) for DH5α (TIANGEN) transformation. After colony screening and identification, positive clones were grown in a 5 mL LB culture medium, and plasmid isolation was performed using a plasmid MiniPrep Kit (Sangon Biotech). DNA from the plasmid was sent to Sangon Biotechnology for sequencing. The concentration of plasmid pMD19T-N34 was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

l-phenylalanine was purchased from Sigma-Aldrich (St. Louis, United States). Styrene, Styrene oxide, (S)-1-phenyl-1,2-ethanediol, and 2-phenylethanol were purchased from Adamas (Shanghai, China). Cinnamic acid and phenylacetaldehyde were obtained from Aladdin (Shanghai, China). The plasmid miniprep kit was purchased from Sangon Biotech (Shanghai, China). All other chemical reagents were of the highest purity available.