The ALK IHC assay included a highly sensitive anti-ALK (D5F3) rabbit monoclonal primary antibody (Ventana Medical Systems Inc), combined with an OptiView Amplification Kit and an OptiView DAB IHC Detection Kit (Ventana Medical Systems Inc). The automatic staining procedure was performed on a Bench- Mark XT slide stainer (Ventana Medical Systems Inc) according to Ventana ALK IHC Assay's instructions. Simultaneously, each case was stained with a matched rabbit monoclonal negative control immunoglobulin G antibody.
Anti alk d5f3 rabbit monoclonal primary antibody
Manufactured by Roche
Sourced in Azerbaijan, United States
The Anti-ALK (D5F3) rabbit monoclonal primary antibody is a laboratory reagent used for the detection of the anaplastic lymphoma kinase (ALK) protein. It is a specific and reliable tool for the identification of ALK expression in biological samples.
Automatically generated - may contain errors
3 protocols using anti alk d5f3 rabbit monoclonal primary antibody
1
Sensitive ALK IHC Assay Protocol
2
ALK Rearrangement Detection by IHC
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Immunohistochemistry (IHC) staining was performed on 4-um-thick slides of FFPE using a Ventana anti-ALK (D5F3) rabbit monoclonal primary antibody combined with the OptiView DAB IHC Detection Kit and the OptiView Amplification Kit (Ventana Medical Systems, Inc. Tucson, AZ). Each patient had a matched negative control slide to assess non-specific background staining and the degree of background staining known to occur as a result of specific tissue elements. The scoring criteria for positive ALK rearrangement was the presence of strong granular cytoplasmic staining in tumor cells (any percentage of positive tumor cells), whereas the absence of strong granular cytoplasmic staining in tumor cells was considered negative ALK rearrangement. All results were confirmed by two experienced pathologists (Y. Z. and J. B. L.).
3
Automated IHC Evaluation of ALK Status
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Immunohistochemical staining was performed on 4 μm thick TMAs sections. Immunohistochemistry (IHC) analysis was performer using the VENTANA anti-ALK (D5F3) Rabbit Monoclonal Primary Antibody, used with the OptiView DAB IHC Detection Kit and OptiView Amplification Kit (Ventana Medical Systems USA), as a fully automated assay on the Ventana BenchMark XT processor. A binary scoring system (positive or negative for ALK status) was adopted to evaluate the staining results, according to the manufacture’s scoring algorithm. The samples were considered positive when tumor cells (any percentage of positive tumor cells) showed a strong granular cytoplasmic staining.
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