For the staining of intracellular Foxp3, cells were incubated with Cy-chrome-labeled anti-CD4 and FITC-labeled anti-CD25 mAbs first [11 (link)]. Then these cells were fixed after washing and stained with anti-mouse Foxp3 mAb, on the basis of the instruction provided by the company (eBioscience, San Diego, CA).
Fascalibur flow cytometry
The FASCalibur is a flow cytometer designed for the analysis of cells and other particles. It is capable of detecting and measuring multiple parameters of individual cells flowing in a fluid stream. The FASCalibur provides accurate and reliable data on cell size, granularity, and fluorescence properties.
3 protocols using fascalibur flow cytometry
Flow Cytometry Analysis of Regulatory T Cells
For the staining of intracellular Foxp3, cells were incubated with Cy-chrome-labeled anti-CD4 and FITC-labeled anti-CD25 mAbs first [11 (link)]. Then these cells were fixed after washing and stained with anti-mouse Foxp3 mAb, on the basis of the instruction provided by the company (eBioscience, San Diego, CA).
Flow Cytometric Analysis of SMO and GLI1
Asthmatic Mice Lymphocyte Assay
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