Protein expression in cells was analyzed by standard Western blot assays. Western blotting was performed using anti-ZFP91 (PA5-41199, Thermo Fisher, 1:2000), anti-hnRNP A1 (11176-1-AP, Proteintech, 1:2000), anti-β-actin (BS6007M, Bioworld, 1:5000), anti-HA (561, MBL, 1:2000), anti-Flag (M185-3L, MBL, 1:2500), anti-PKM1 (#7067, CST, 1:1000), and anti-PKM2 (15822-1-AP, Proteintech, 1:1500). Band intensity was quantified using ImageJ software (NIH, Bethesda).
Anti β actin bs6007m
Manufactured by Bioworld Technology
Sourced in United States
Anti-β-actin (BS6007M) is a monoclonal antibody that specifically targets the beta-actin protein. Beta-actin is a ubiquitous cytoskeletal protein found in all eukaryotic cells and is often used as a reference or loading control in various biological experiments. This antibody can be used to detect and quantify beta-actin levels in samples.
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Lab products found in correlation
2 protocols using anti β actin bs6007m
1
Western Blot Analysis of Protein Expression
2
Investigating YAP, Cyr61, and Apoptosis Signaling
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The primary antibodies, anti-YAP (sc-15407) was purchased from Santa-cruz (USA), anti-Cyr61 (11952S), Bcl-2(#2870S) and BAX (#2772) were from Cell signaling technology (CST, USA), and anti-βactin (BS6007M) was purchased from Bioworld (USA). The secondary antibodies, horseradish peroxidaseconjugated goat anti-rabbit IgG (BS13278) and horseradish peroxidase-conjugated goat anti-mouse IgG (#7076S) were purchased from CST (USA), and horseradish peroxidase-conjugated goat anti-rabbit IgG (BS13278) was purchased from Bioworld (USA). The secondary antibody, fluorophore-labeled goat antirabbit IgG (ab350077) was purchased from abcam (UK). PTX and VP used in chemotherapeutic drug experiments were purchased from Sigma (USA). YAP-siRNA (sc-38637) was purchased from Santa-cruz (USA). The plasmids used in transfection were purchased from Genloci Biotechnoligies Inc. (China). Control experiments were performed using one set of cells, and then repeated at least three times.
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