Following short-term concentration–response treatments, 96-well plates were fixed with methanol and stained with first antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) at 1:5000 dilution and second antibody F(ab’)2-Goat anti-human IgG Fc Cross-Adsorbed Secondary Antibody, HRP (Invitrogen #A24476, Carlsbad, CA, USA) or Goat F(ab’)2 Anti-Human IgG—Fc (HRP), preabsorbed (Abcam #ab98595, Cambridge, UK) at 1:2000 dilution. Next, infected cells were visualized by staining with DAB substrate, BrightDAB kit (Immunologic#BS04-110, Duiven, The Netherlands). The ImmunoSpot Series 5 UV Analyzer (CTL Europe GmbH, Bonn, Germany) was used to evaluate the number of single SARS-CoV-2 spike-positive cells per well. Representative images of stained 96-well plates are shown in a previous publication [57 (link)].
Sars cov 2 spike chimeric monoclonal antibody
Manufactured by Sino Biological
Sourced in China
The SARS-CoV-2 spike chimeric monoclonal antibody is a laboratory tool used for research purposes. It is a recombinant antibody that binds to the spike protein of the SARS-CoV-2 virus. This antibody can be used in various research applications, such as assay development, protein interaction studies, and COVID-19 research.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 goat anti human igg h l, by Thermo Fisher Scientific (1 mentions)
8 well chamber slide, by Thermo Fisher Scientific (1 mentions)
Axio vert a1, by Zeiss (1 mentions)
Alexa fluor 488 goat anti, by Thermo Fisher Scientific (1 mentions)
3 protocols using sars cov 2 spike chimeric monoclonal antibody
1
SARS-CoV-2 Spike Protein Quantification
2
SARS-CoV-2 Spike Protein Immunofluorescence Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In longer-term SARS-CoV-2-infected and PI-treated cultures, following cell splitting and treatment, replicate cell cultures were seeded in 8-well chamber slides (Thermo Fisher Scientific, Rochester, NY, USA). The next day, cells were fixed, and virus was inactivated by submersion into methanol for 20 min. Chamber slides were washed twice with PBS-Tween and then incubated with primary antibody, SARS-CoV-2 spike chimeric monoclonal antibody (no. 40150-D004; Sino Biological, Beijing, China), diluted 1:1,000 in PBSK for 2 h at room temperature. Following 2 washes with PBS-Tween, chamber slides were incubated with secondary antibody, Alexa-Fluor 488 goat anti-human IgG(H+L) (no. A-11013; Invitrogen, Paisley, UK), diluted 1:500 and Hoechst 33342 (Invitrogen) diluted 1:1,000 in PBS-Tween for 20 min at room temperature. The percentage of SARS-CoV-2 spike protein-positive cells was evaluated by fluorescence microscopy (Axio Vert.A1; Zeiss, Jena, Germany), assigning the following designations: 0% infected cells (no cells infected), single infected cells, and 10% to 90% infected cells (in steps of 10%). The images were acquired with ZEN 3.0 software.
3
SARS-CoV-2 Spike Protein Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
During and following longer-term treatment assays, chamber slides were fixed with methanol and stained with first antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) at 1:1000 dilution and second antibody Alexa Fluor 488 goat anti-human immunoglobulin G (IgG) (H + L) (Invitrogen #A-11013, Paisley, UK) at 1:500 dilution combined with Hoechst 33342 (Invitrogen, Paisley, UK) at 1:1000 dilution. Fluorescence microscopy (ZEISS Axio Vert.A1, Jena, Germany) was used to evaluate the percentages of SARS-CoV-2 spike-positive cells, using the following designations: 0% positive cells (no cells infected), single positive cells, and 10 to 90% positive cells, in steps of 10%.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!