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Seeplex rv7 kit

Manufactured by Seegene

The Seeplex® RV7 kit is a multiplex real-time PCR assay designed for the simultaneous detection of seven common respiratory viruses: influenza A, influenza B, respiratory syncytial virus A, respiratory syncytial virus B, parainfluenza virus 1, parainfluenza virus 3, and human metapneumovirus. The kit is intended for use in clinical laboratories to aid in the diagnosis of respiratory viral infections.

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3 protocols using seeplex rv7 kit

1

Viral and Bacterial Co-infections in Hospitalized Patients

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Viral respiratory infection was diagnosed by PCR from combined pharyngeal and nasopharyngeal swabs introduced into UTM tubes and transported to the laboratory. RNA extraction was by the easyMAG® system (BioMérieux, Marcy-l'Étoile, France). RSV was diagnosed by the Simplexa™ Flu A/B & RSV kit (DiaSorin) or the Seeplex® RV7 kit (Seegene).
All positive cultures (blood, sputum, urine and others) from the first seven days of hospitalization were extracted for identification of bacterial or fungal super-infections. The list was reviewed independently by two internal medicine physicians to exclude positive cultures that were considered to be contaminants.
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2

Molecular Diagnosis of Viral Respiratory Infections

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The diagnosis of viral respiratory infection was done by PCR. Samples were collected by combined pharyngeal and nasopharyngeal swabs that were inoculated into UTM tubes and immediately transported to the laboratory. RNA extraction was done using the easyMAG® system (BioMérieux, Marcy-l'Étoile, France). The diagnosis of SARS-CoV-2 was done by RT-PCR using mostly the Allplex™ 2019-nCoV Assay (Seegene). The diagnosis of Influenza A&B and RSV was done using the Simplexa™ Flu A/B & RSV kit (DiaSorin) or the Seeplex® RV7 kit (Seegene).
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3

Viral Infection Diagnosis by PCR

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The diagnosis of the different viral infections was undertaken by PCR from nasopharyngeal and pharyngeal swabs. RNA extraction was done using the easyMAG® system (BioMérieux, Marcy Étoile, France). The diagnosis of SARS-CoV-2 was done by RT-PCR using mostly the Allplex™ 2019-nCoV Assay (Seegene). The diagnosis of Influenza A and B and RSV was done using the Simplexa™ Flu A/B & RSV kit (DiaSorin) or the Seeplex® RV7 kit (Seegene).
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