LC-MS analysis used a 4000 QTRAP triple quadrupole mass spectrometer (Applied Biosystems/Sciex), coupled to an Acquity UPLC (Waters) as described by Roberts et al. (2012) (link), except that an Aquity UPLC (Waters) was used and multiple reaction monitoring parameters were added as outlined in Table S5. Formic acid, ammonium acetate, LC-MS-grade solvents and valine-d8 were from Sigma-Aldrich. Dried aqueous fractions from HeLa and 2FTGH cells were prepared for LC-MS analyses by the addition of 100 µl of 74.9 : 24.9 : 0.2 (by vol.) acetonitrile/methanol/Formic acid containing the stable isotope-labelled internal standard valine-d8. Samples were vortexed, sonicated and the supernatants were injected directly.
4000 qtrap triple quadrupole mass spectrometer
Manufactured by Thermo Fisher Scientific
Sourced in Japan
The 4000 QTRAP triple quadrupole mass spectrometer is a high-performance analytical instrument designed for quantitative and qualitative analysis of small molecules and peptides. It features a triple quadrupole configuration, providing enhanced sensitivity and selectivity for targeted analyte detection and measurement.
Automatically generated - may contain errors
Lab products found in correlation
Acquity uplc, by Waters Corporation (1 mentions)
Lc ms grade solvents, by Merck Group (1 mentions)
Aquity uplc, by Waters Corporation (1 mentions)
Valine d8, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Lc 20ab pump, by Shimadzu (1 mentions)
Sil 20ac ht autosampler, by Shimadzu (1 mentions)
Ultra c18 column, by Restek (1 mentions)
3 protocols using 4000 qtrap triple quadrupole mass spectrometer
1
LC-MS Analysis of HeLa and 2FTGH Cells
2
HPLC-MS/MS Analysis of Compounds
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The HPLC system comprises of an LC-20AB pump, a CTO-20A column oven, and an SIL-20AC/HT autosampler (Shimadzu Corporation, Japan) coupled with a 4000Qtrap triple quadrupole mass spectrometer with a heated electrospray ionization source (Applied Biosystems, Foster City, CA, USA). Collection and processing of data was accomplished with Analyst 1.4.2 software.
3
Liquid Chromatography-Tandem Mass Spectrometry Analysis
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Extracted sediment samples were analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS). This was performed on an Agilent 1200LC liquid chromatograph coupled to a 4000 QTrap triple-quadrupole mass spectrometer (Applied Biosystems/MDS Sciex) operated in negative electrospray ionization mode.
Analyte separation was performed using a Restek Ultra C18 column (50 mm × 2.1 mm, 3 µm; Restek, Bellefonte, PA, USA). The optimized instrumental parameters and HPLC mobile phase gradient are described in detail by Guo et al. (2012) . An overview of the MS/MS settings and multiple reaction monitoring (MRM) target ions are presented as Table 2.
Analyte separation was performed using a Restek Ultra C18 column (50 mm × 2.1 mm, 3 µm; Restek, Bellefonte, PA, USA). The optimized instrumental parameters and HPLC mobile phase gradient are described in detail by Guo et al. (2012) . An overview of the MS/MS settings and multiple reaction monitoring (MRM) target ions are presented as Table 2.
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