Anti human cd14 percp cy5

Flow cytometry was conducted as previously described (Hou et al., 2016 (link)). The antibodies used in this study were as follows: Anti-Human CD14 PerCP-Cy5.5, mouse IgG1κ isotype control PerCP-Cy5.5, anti-mouse CD11b FITC, rat IgG2a K isotype control FITC, anti-mouse TIM-3 PE, and rat IgG2α K isotype control PE (all obtained from eBioscience, San Diego, CA, USA), anti-human Tim-3-PE, and rat IgG_2A isotope control-PE (both obtained from R&D Systems). The cells were detected and analyzed using a FACS Canto II flow cytometer (BD Biosciences, San Jose, CA, USA), and the gates for positive cells were defined using the isotype controls.

AS-IV powder (C₄₁H₆₈O₁₄, molecular weight: 784.9702, purity > 98%) was purchased from Shanghai Ronghe Co. (Shanghai, China). The following antibodies were purchased from eBiosciences (CA, USA): anti-human CD206 PE, anti-human CD86 PE, anti-human CD14 PerCP/Cy5.5, anti-mouse CD45 v450, anti-mouse CD11b FITC, anti-mouse F4/80 PE, anti-mouse CD206 Alexa-647, and anti-mouse iNOS APC. Antibodies against total and phosphorylated AMPKα, arginase 1 (Arg-1), CD31, and vascular endothelial growth factor A (VEGFA) were purchased from Cell Signaling Technology (MA, USA). IL4, IL13, IFN-γ, and LPS were purchased from Peprotech (NJ, USA). Phorbol 12-myristate 13-acetate (PMA) was purchased from Liankebio (Hangzhou, China). JetPrime transfection agent was obtained from Polyplus (NY, USA).