α zearalenol α zol

The mycotoxins α-zearalenol (α-ZOL) and β-zearalenol (β-ZOL) were purchased from Sigma-Aldrich. Native and chemically modified cyclodextrins, including β-cyclodextrin (BCD), (2-hydroxypropyl)-β-cyclodextrin (HPBCD), randomly methylated-β-cyclodextrin (RAMEB), heptakis-2,6-di-O-methyl-β-cyclodextrin (DIMEB), sulfobutylated β-cyclodextrin (SBBCD), carboxymethyl-β-cyclodextrin (CMBCD), and (2-hydroxy-3-N,N,N-trimethylamino)propyl-β-cyclodextrin (QABCD; or quaternary ammonium β-cyclodextrin), were obtained from Cyclodextrin Research & Development Laboratory Ltd. Stock solutions of ZOLs (5000 μM) were prepared in ethanol (Reanal, spectroscopic grade) and stored at −20 °C protected from light.

Zearalenone (ZEN), α-zearalenol (α-ZOL), and β-zearalenol (β-ZOL) standards were purchased from Sigma-Aldrich (St. Louis, MO, USA) and stored at −20 °C. Stock solutions of ZEN (1 mg/L), α-ZOL (1 mg/L), and β-ZOL (1 mg/L) were prepared by dissolving them in 100% methanol. The standard solutions for high-performance liquid chromatography (HPLC) calibration were prepared by diluting the stock solution with a methanol/water (50/50, v/v) mixture. Other chemicals such as urea, sodium dodecyl sulfate (SDS), m-periodate, ploymyxin B, protease E, and lipase were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). The Lactobacillus biochemical identification Kit and De Man Rogosa and Sharpe (MRS) medium were provided by Huankai Microbial Science & Technology Co., Ltd. (Guangzhou, China). All the other chemicals used in this experiment were of chromatographic or analytical grade.