A14031

Spinal cord tissue sections were depara nized twice with xylene for 15 min each time, and treated with graded alcohol. Next, the sections were washed with PBS for three times. Antigen retrieval was performed with antigen retrieval solution (9 ml of citric acid buffer, 41 ml of sodium citrate buffer and 450 ml of distilled water) for 10 min at a high-temperature. After washing three times with PBS, the sections were incubated with 1% BSA (Sangon, China) for 15 min. Primary antibody incubation was performed at 4°C overnight and followed by incubation with secondary antibody for 1 h at room temperature. DAPI (Solarbio, China) was used to counterstain nuclei. Finally, sections were treated with anti-uorescence quencher (Solarbio, China). The staining was observed under microscope. Below is the information of antibodies, MBP antibody (1:50 dilution, A11162, Abclonal, China); iNOS antibody (1:50 dilution, A14031, Abclonal, China); Arg1 antibody (1:50 dilution, A4923, Abclonal, China); HPGD antibody (1:50 dilution, A5024, Abclonal, China); Iba-1 antibody (1:100 dilution, ab283319, Abcam, UK); Goat anti-rabbit IgG (Cy3) (1:200 dilution, ab6939, Abcam, UK); and Goat anti-mouse IgG (FITC) (1:200 dilution, ab6785, Abcam, UK).