AFM experiments were always done in liquid, imaging was performed at the SPM@ISMN facility in Bologna [27, (link)28] (link) using a Multimode VIII (Bruker, Santa Barbara, CA, US) and at the Partnership for Soft Condensed Matter (PSCM) in Grenoble using a Cypher S (Asylum Research, Santa Barbara, CA, US). In the first case images were collected in peakforce tapping using SNL Bruker cantilevers with nominal spring constant of 0.24 N/m and 2-10 nm curvature radius, in the second one Olympus BL-AC40TS cantilevers were chosen to perform tapping mode imaging. Images were processed with Gwyddion (D Nec ˇas & P Klapetek. ''Gwyddion: an open-source software for SPM data analysis, by simply plane-fitting. SLBs and SLBs + SPIONs were prepared according to the previously described protocol.
Cantilevers
Manufactured by Bruker
Sourced in Germany
Cantilevers are a core component in atomic force microscopy (AFM) instruments. They are small, flexible beams that are used to detect and measure the interactions between a probe tip and a sample surface at the nanoscale level. Cantilevers are designed to respond to very small forces, allowing for high-resolution imaging and measurement of surface topography and properties.
Automatically generated - may contain errors
4 protocols using cantilevers
1
Atomic Force Microscopy of Supported Lipid Bilayers
2
Atomic Force Microscopy of Self-Assembled Spidroins
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For atomic
force electron microscopy (AFM) measurements, self-assembled recombinant
spidroin dispersions were diluted to 0.1 mg/mL by deionized water.
10 μL of the aqueous solution was then added onto a clean mica
surface for coating for 120 s, followed by purging with nitrogen gas.
The morphologies of the nanoparticles and nanofibrils were observed
by an ICON AFM fast scanning system (Bruker ICON). A silicon tip with
a nominal spring constant of 0.4 N/m (ScanAsyst-Air, Bruker) was used
for the AFM measurements. The mechanical properties of self-assembled
spidroins was measured by a cantilever with a spring constant of ≈34.32
N m–1 and a tip radius of 8 nm. Of note, the spring
constant of the cantilevers (Bruker, Germany) was calibrated by a
sapphire wafer. The morphological and mechanical data of self-assembled
spidroins were processed by the software NanoScope Analysis 1.8.
force electron microscopy (AFM) measurements, self-assembled recombinant
spidroin dispersions were diluted to 0.1 mg/mL by deionized water.
10 μL of the aqueous solution was then added onto a clean mica
surface for coating for 120 s, followed by purging with nitrogen gas.
The morphologies of the nanoparticles and nanofibrils were observed
by an ICON AFM fast scanning system (Bruker ICON). A silicon tip with
a nominal spring constant of 0.4 N/m (ScanAsyst-Air, Bruker) was used
for the AFM measurements. The mechanical properties of self-assembled
spidroins was measured by a cantilever with a spring constant of ≈34.32
N m–1 and a tip radius of 8 nm. Of note, the spring
constant of the cantilevers (Bruker, Germany) was calibrated by a
sapphire wafer. The morphological and mechanical data of self-assembled
spidroins were processed by the software NanoScope Analysis 1.8.
3
Surface Characterization by Tapping Mode AFM
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Scanning of the surfaces was performed using tapping mode atomic force microscopy (AFM; Nanowizard 3 from JPK). Cantilevers (Bruker) with a force constant of 3 N m–1 and a resonance frequency of 75 kHz were used.
4
Mechanical Characterization of Hydrogels
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