Penicillium streptomycin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Penicillium-streptomycin is a laboratory product that contains a combination of the antibiotic compounds penicillin and streptomycin. It is commonly used in cell culture applications to prevent bacterial contamination.

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Lab products found in correlation

Expifectamine 293, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Vero 76, by American Type Culture Collection (1 mentions) Vero ccl81, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Streptomycin (24872 citations) Penicillium (2 citations)

2 protocols using penicillium streptomycin

Cell Culture and Transfection Protocol

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HEK293T and Vero 76 cells (both from ATCC) were cultured in Dulbecco's modified Eagle's medium (DMEM) with GlutaMAX (Gibco) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich). Cell lines were tested for mycoplasma contamination after receipt, before expansion and cryopreservation. For studies including nonhuman primate samples, Vero 76 and Vero CCL81 cells (both from ATCC) were cultured in DMEM (Gibco) containing 2% HyClone fetal bovine and 100 U ml -1 penicillium-streptomycin (Gibco). Expi293F cells were grown in Expi293 medium and transiently transfected using Expi-Fectamine293 (all from Thermo Fisher Scientific).

Vogel A.B., Kanevsky I., Che Y., Swanson K.A., Muik A., Vormehr M., Kranz L.M., Walzer K.C., Hein S., Güler A., Loschko J., Maddur M.S., Ota-Setlik A., Tompkins K., Cole J., Lui B.G., Ziegenhals T., Plaschke A., Eisel D., Dany S.C., Fesser S., Erbar S., Bates F., Schneider D., Jesionek B., Sänger B., Wallisch A.K., Feuchter Y., Junginger H., Krumm S.A., Heinen A.P., Adams-Quack P., Schlereth J., Schille S., Kröner C., de la Caridad Güimil Garcia R., Hiller T., Fischer L., Sellers R.S., Choudhary S., Gonzalez O., Vascotto F., Gutman M.R., Fontenot J.A., Hall-Ursone S., Brasky K., Griffor M.C., Han S., Su A.A.H., Lees J.A., Nedoma N.L., Mashalidis E.H., Sahasrabudhe P.V., Tan C.Y., Pavliakova D., Singh G., Fontes-Garfias C., Pride M., Scully I.L., Ciolino T., Obregon J., Gazi M., Carrion R J.r., Alfson K.J., Kalina W.V., Kaushal D., Shi P.Y., Klamp T., Rosenbaum C., Kuhn A.N., Türeci Ö., Dormitzer P.R., Jansen K.U, & Sahin U. (2021). BNT162b vaccines protect rhesus macaques from SARS-CoV-2. Nature, 592(7853).

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Culturing Human Colorectal Adenocarcinoma Cells

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P a s s a g e 1 9 o f h u m a n c o l o r e c t a l adenocarcinoma (HT-29) was used in this study and was grown in the Roswell Park Memorial Institute (RPMI)-1640 medium. The medium was supplemented with 10% fetal bovine serum (FBS) (Gibco™, USA) and 1% penicillium-streptomycin (10,000 U/mL, Gibco™, USA) to ensure cell growth and viability. The cells were incubated in a humidified atmosphere with 5% carbon dioxide at 37 °C for growth and maintenance.

Zain S.N.D.M., & Omar W.A.W. (2021). Phyllanthus debilis Methanolic Extract Reduces the Viability of Human Colorectal Adenocarcinoma (HT-29) Cells and Increases LINE-1 and Alu DNA Methylation. Pertanika journal of tropical agricultural science, 45(1), 25-36.

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