Purepoint laser

Manufactured by Alcon

Sourced in United States

The Purepoint Laser is a high-performance laser system designed for a variety of laboratory applications. It features a compact and durable design, providing a stable and consistent output. The Purepoint Laser is capable of delivering precise and uniform illumination, making it a versatile tool for various scientific and research purposes.

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Lab products found in correlation

Mm af morphometric analysis software, by Leica (1 mentions) Fitc dextran, by Leica (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Tcs spe confocal microscope, by Leica (1 mentions)

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Purepoint (2 citations)

2 protocols using purepoint laser

Laser-Induced Choroidal Neovascularization

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Choroidal neovascularization (CNV) was induced by laser burns as previously described with adaptations (Schoors et al., 2014 ) using a Purepoint Laser (Alcon, Fort Worth, USA). Ten or eight impacts (for choroidal endothelial cell isolation or immunostaining, respectively) rupturing the Bruch's membrane were made around the optical nerve using laser diameter 100 mm, power 0.320 W and exposure time 0.05 s in both eyes. At day 7, at the height of the angiogenic response (Lambert et al., 2013) , mice were euthanized by cervical dislocation and the eyes were enucleated.

Rohlenova K., Goveia J., García-Caballero M., Subramanian A., Kalucka J., Treps L., Falkenberg K.D., de Rooij L.P.M.H., Zheng Y., Lin L., Sokol L., Teuwen L.A., Geldhof V., Taverna F., Pircher A., Conradi L.C., Khan S., Stegen S., Panovska D., De Smet F., Staal F.J.T., Mclaughlin R.J., Vinckier S., Van Bergen T., Ectors N., De Haes P., Wang J., Bolund L., Schoonjans L., Karakach T.K., Yang H., Carmeliet G., Liu Y., Thienpont B., Dewerchin M., Eelen G., Li X., Luo Y, & Carmeliet P. (2020). Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis. Cell metabolism, 31(4).

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Laser-Induced Choroidal Neovascularization in Mice

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Choroidal neovascularization (CNV) was induced by laser burns using a Purepoint Laser (Alcon, Fort Worth, USA)^{67 (link)}. Ten or eight impacts (for choroidal endothelial cell isolation or histological analysis, respectively) rupturing the Bruch’s membrane were made around the optical nerve using a laser diameter of 100 μm, power 0.320 W, and exposure time of 0.05 s in both eyes. On day 7, at the height of the angiogenic response^{56 (link)}, mice were euthanized by cervical dislocation (three mice (six eyes) per condition), and the eyes (for histological analysis) were enucleated 10 min after retrobulbar injection with Fluorescein isothiocyanate (FITC)-conjugated dextran (Mr 2,000,000) (Sigma-Aldrich) and fixed in 2% paraformaldehyde. Choroids were dissected, flat-mounted (ProLong Gold antifade reagent, Thermo Fisher Scientific), and imaged using a Leica TCS SPE confocal microscope (Leica Microsystems). Analysis of the neovascular area was performed with the Leica MM AF morphometric analysis software (Leica Microsystems) and expressed as the FITC-dextran positive area in percent of the total CNV lesion area. This procedure was repeated for 4–5 independent replicate experiments, each: three mice (six eyes) per condition.

Sokol L., Cuypers A., Truong A.C., Bouché A., Brepoels K., Souffreau J., Rohlenova K., Vinckier S., Schoonjans L., Eelen G., Dewerchin M., de Rooij L.P, & Carmeliet P. (2023). Prioritization and functional validation of target genes from single-cell transcriptomics studies. Communications Biology, 6, 648.

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