Anti human cd71 apc

Human PBMCs (1 ​× ​10⁷/mL) were resuspended in 100 ​μL FACS buffer (DPBS contained 1% heat inactivated FBS) with 1 ​× ​10⁶ ​cells. PBMCs were stained with the following fluorescein-labeled antibodies: anti-human CD19 PE-Cy7 (Cat^#: IM3628, Beckman, USA), anti-human CD21 FITC (Cat^#: 561372, BD Biosciences, USA), anti-human CD27 BB700 (Cat^#: 566449, BD Biosciences, USA), anti-human CD71 APC (Cat^#: 334108, Biolegend, USA), anti-human IgD PE (Cat^#: 12-9868-42, Invitrogen, USA), anti-human IgG APC-H7 (Cat^#: 561297, Biolegend USA) for 30 ​min. Then, immunophenotype of MBCs was detected using BD FACS LSR Fortessa flow cytometer (BD Biosciences, USA). Data were analyzed using FlowJo software (Tree Star, USA).