The histoscore for the immunoreactivity of both Axin2 and Snail in the surgical margins of OSCC was calculated according to the staining intensity and percentage of positive cells using the weighted histoscore method (26 (link)). Patients were subdivided into two groups according to the histoscore: low (histoscore 0–100) and high (histoscore 101–300) expression groups.
Real envision hrp rabbit mouse detection system
The Real Envision HRP Rabbit/Mouse detection system is a laboratory equipment product manufactured by Agilent Technologies. It is designed to detect the presence of rabbit or mouse proteins in biological samples using a horseradish peroxidase (HRP) enzyme-based detection method.
Lab products found in correlation
2 protocols using real envision hrp rabbit mouse detection system
Immunohistochemical Analysis of Axin2 and Snail Expression in OSCC
The histoscore for the immunoreactivity of both Axin2 and Snail in the surgical margins of OSCC was calculated according to the staining intensity and percentage of positive cells using the weighted histoscore method (26 (link)). Patients were subdivided into two groups according to the histoscore: low (histoscore 0–100) and high (histoscore 101–300) expression groups.
Immunohistochemistry for HIF-1α Expression
As described in our previous study, the weighted histoscore method was used to score HIF-1a expression according to staining intensity and the percentage of positively stained cells (Zhang et al., 2014) . The final score was calculated as follows:
final score¼ (0
The samples were subdivided into three groups, weak (histoscores 0e100), moderate (histoscores 101e200), and high HIF-1aeexpressing group (histoscores 201e300), based on the final histoscore.
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