Cd162 2ph1

Manufactured by BD

The CD162 (2PH1) is a laboratory equipment designed for cell analysis. It is a dual-channel flow cytometer capable of detecting and analyzing various cell populations based on their physical and fluorescent properties. The core function of the CD162 (2PH1) is to enable researchers and scientists to perform quantitative and qualitative analysis of cells, including identification, enumeration, and characterization.

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Lab products found in correlation

Fixation permeabilization working solution, by Thermo Fisher Scientific (1 mentions) Facsuite software, by BD (1 mentions) Tnf α mp6 xt22, by BioLegend (1 mentions) Ionomycin, by Nacalai Tesque (1 mentions) Phorbol myristate acetate (pma), by Nacalai Tesque (1 mentions) Golgiplug, by BD (1 mentions) Cd103 2e7, by BioLegend (1 mentions) Cd127 a7r34, by BioLegend (1 mentions) Streptavidin, by Thermo Fisher Scientific (1 mentions) Cd45.1 a20, by Cytek Biosciences (1 mentions) Cd69 h1.2f3, by BioLegend (1 mentions) Cd8β h35 17, by Thermo Fisher Scientific (1 mentions) Cd45.2 104, by Cytek Biosciences (1 mentions) Klrg1 2f1 klrg1, by BioLegend (1 mentions) Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions) Facscelesta, by BD (1 mentions)

2 protocols using cd162 2ph1

Multicolor Flow Cytometry for Murine Lymphocytes

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The following antibodies were used for flow cytometric analysis of mouse lymphocytes:
Anti-CD3e (145-2C11), CCR5 (C34-3448), IFN-γ (XMG1.2), IL-2 (JES6-5H4), IL-17 (TC11-18H10), CD29 (Ha2/5), CD49d (9C10), and CD162 (2PH1) antibodies were purchased from BD Pharmingen. Anti-CD4 (RM4-5), CD8 (53–6.7), CD44 (IM7), CD62L (MEL-14), CXCR3 (CXCR3-173), CCR4 (2G12), and TNF-α (MP6-XT22) antibodies were from Biolegend. Anti-CD25 (pc61), Foxp3 (FJK-16s), CCR9 (eBioCW-1.2), and IL-4 (11B11) antibodies were from eBioscience. Anti-CCR10 antibody (248918) was purchased from R&D systems. In order to stain cytokines, splenocytes were stimulated with 50ng/mL PMA (Nakarai Tesque), 1μg/mL ionomycin (Nakarai Tesque) and a protein transport inhibitor, BD Golgi plug (BD Pharmingen) for 4 hours before harvesting cells. After cell surface staining, cells were fixed and permeabilized with Fixation/Permeabilization working solution (eBioscience) and intracellular antigens were stained. Flow cytometric analysis was carried out using a FACS Verse with FACSuite software (BD Biosciences) and Flow Jo (FlowJo, LLC).

Mitagami Y., Yasunaga J.I., Kinosada H., Ohshima K, & Matsuoka M. (2015). Interferon-γ Promotes Inflammation and Development of T-Cell Lymphoma in HTLV-1 bZIP Factor Transgenic Mice. PLoS Pathogens, 11(8), e1005120.

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Multi-parameter analysis of splenic and kidney cells

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Single cell suspension from spleen and kidney was incubated with FcR blocker (clone 2.4G2, generated in the lab). Cells were typically stained with fluorescence labeled streptavidin (Thermo Fisher), CD8β (H35-17.2, eBioscience), CD162 (2PH1, BD), CD45.1 (A20, Tonbo), CD45.2 (104, Tonbo) and the following antibodies from BioLegend, CD127 (A7R34), KLRG1 (2F1/KLRG1), CD69 (H1.2F3), CD103 (2E7), CD90.1 (OX-7), CD43 (1B11), CXCR3 (CXCR3-173), mouse P-selectin/hIgG1 Fc chimera protein and mouse E-selectin/hIgG1 Fc chimera protein. For E/P-selectin binding experiments, fluorescence labeled anti-human IgG secondary antibody was purchased from Jackson ImmunoResearch. In some experiments, Fixable Viability Dye eFluor 506 (eBioscience) was used to identify live cells. Washed and fixed samples were analyzed by BD LSRII or BD FACSCelesta and analyzed by FlowJO (TreeStar) software.

Ma C., Mishra S., Demel E.L., Liu Y, & Zhang N. (2016). TGF-β controls the formation of kidney-resident T cells via promoting effector T cell extravasation. Journal of immunology (Baltimore, Md. : 1950), 198(2), 749-756.

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