pEBG-GST-CAPK plasmids encoding CAPK wild type (WT), kinase dead (KD), and CTD truncation (1–291), as well as pEGFP-CAPK plasmids encoding CAPK WT, KD, R272A, and CTD truncation (1–291) were described in [2 (link),3 (link)]. pCIG-HA-KIF3A was described in [20 (link)].
KIF3A-phospho-Thr672 antibody was generated in rabbits against keyhole limpet hemocyanin-coupled phospho-KIF3A peptide RPR[pT]SKGKARPKTGC at GenScript (Piscataway, NJ, USA). Phosphopeptide-specific antibodies were affinity-purified through a positive selection over phosphopeptide antigens followed by negative selections over non-phosphopeptide antigens. GST-tag (B-14) mouse monoclonal (sc-138) and HA-tag (12CA5) mouse monoclonal (sc-57592) antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA). KIF3A (D7G3) rabbit monoclonal (#8507) and HA-tag (C29F4) rabbit monoclonal (#3724) antibodies were from Cell Signaling Technology (Danvers, MA, USA). Arl13B rabbit polyclonal antibody (17711-1-AP) was from Proteintech (Rosemont, IL, USA). Goat anti-rabbit IgG (Alexa Fluor 594) preadsorbed antibody (ab150084) was from Abcam (Cambridge, MA, USA).
KIF3A-phospho-Thr672 antibody was generated in rabbits against keyhole limpet hemocyanin-coupled phospho-KIF3A peptide RPR[pT]SKGKARPKTGC at GenScript (Piscataway, NJ, USA). Phosphopeptide-specific antibodies were affinity-purified through a positive selection over phosphopeptide antigens followed by negative selections over non-phosphopeptide antigens. GST-tag (B-14) mouse monoclonal (sc-138) and HA-tag (12CA5) mouse monoclonal (sc-57592) antibodies were from Santa Cruz Biotechnology (Dallas, TX, USA). KIF3A (D7G3) rabbit monoclonal (#8507) and HA-tag (C29F4) rabbit monoclonal (#3724) antibodies were from Cell Signaling Technology (Danvers, MA, USA). Arl13B rabbit polyclonal antibody (17711-1-AP) was from Proteintech (Rosemont, IL, USA). Goat anti-rabbit IgG (Alexa Fluor 594) preadsorbed antibody (ab150084) was from Abcam (Cambridge, MA, USA).