Normal human serum and FD-depleted sera were obtained from Complement Technology, Tyler, TX. Human and mouse serum samples were stored at 270°C and were thawed only one time prior to use. Human serum deficient in MASP-2 and MASP-1/3 for these studies were kindly provided by Dr. P. Garred (Copenhagen, Demark) and Dr. S. Thiel (Aarhus, Denmark), respectively. We have established a colony of FD−/−, MASP-1/3−/−, and MASP-2−/− miceonC57BL/6background.Sera fromWT,FD−/−,MASP-1/3−/−, and MASP-2−/− mice were obtained through retro-orbital bleeding according to the approved protocol by the Institutional Animal Care and Use Committee. All mice used in this study were on C57BL/6 background. WT C57BL/6 mice were also purchased from The Jackson Laboratory to obtain serum for various studies. All mice were kept in our barrier facility and fed breeder chow provided by the Center for Laboratory Animal Care, University of Colorado Anschutz Medical Campus.
Fd depleted sera
Manufactured by Complement Technology
FD-depleted sera is a specialized laboratory product designed to remove Fibrin/Fibrinogen Depletion (FD) from serum samples. This product is intended for use in research applications where the removal of FD from serum is necessary for experimental purposes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fd depleted sera
1
Obtaining Sera from Genetically Engineered Mice
2
Obtaining Sera from Genetically Engineered Mice
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Normal human serum and FD-depleted sera were obtained from Complement Technology, Tyler, TX. Human and mouse serum samples were stored at 270°C and were thawed only one time prior to use. Human serum deficient in MASP-2 and MASP-1/3 for these studies were kindly provided by Dr. P. Garred (Copenhagen, Demark) and Dr. S. Thiel (Aarhus, Denmark), respectively. We have established a colony of FD−/−, MASP-1/3−/−, and MASP-2−/− miceonC57BL/6background.Sera fromWT,FD−/−,MASP-1/3−/−, and MASP-2−/− mice were obtained through retro-orbital bleeding according to the approved protocol by the Institutional Animal Care and Use Committee. All mice used in this study were on C57BL/6 background. WT C57BL/6 mice were also purchased from The Jackson Laboratory to obtain serum for various studies. All mice were kept in our barrier facility and fed breeder chow provided by the Center for Laboratory Animal Care, University of Colorado Anschutz Medical Campus.
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