UV-vis absorbance spectra were measured at 20 °C on a JASCO V-750 spectrophotometer equipped with a JASCO PAC-743R thermocontrolled cell changer and a JASCO CTU-100 thermocirculator. Melting temperatures were determined by measuring changes in absorbance at 260 nm as a function of temperature. Absorbance was recorded from 15 to 90 °C at a rate of 1 °C min−1. All samples were prepared in a total volume of 120 μL containing 2.5 μM oligonucleotide and 10 mM Na cacodylate buffer (various pH).
Ctu 100 thermocirculator
Manufactured by Jasco
The CTU-100 is a thermocirculator designed for temperature control applications. It features a digital temperature display, adjustable temperature range, and a robust construction for reliable performance.
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Lab products found in correlation
2 protocols using ctu 100 thermocirculator
1
Thermal Stability of Oligonucleotides
2
Oligonucleotide Melting Temperature Determination
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Melting temperatures were determined by measuring changes in absorbance at 295 nm as a function of temperature on a JASCO V-750 spectrophotometer equipped with a JASCO PAC-743R thermocontrolled cell changer and a JASCO CTU-100 thermocirculator. Absorbance was recorded from 15 °C to 90 °C at a rate of 1 °C/min. The melting samples were denatured at 95 °C for 5 min and annealed slowly to room temperature then stored at 4 °C until experiments were initiated. All melting samples were prepared in a total volume of 120 μL containing 10 μM oligonucleotide and
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