Hepg2

The human LC cell HepG2, Hep3B, and Huh-7 cell lines were purchased from China Infrastructure of Cell Line Resources (School of Basic Medicine Peking Union Medical College, China) and cultured in Dulbecco’s modified Eagle medium containing 10% (v/v) FBS and 100 U/ml streptomycin/penicillin at 37°C and 5% CO₂. Cell Counting Kit-8 (CCK-8) reagent was purchased from Dojindo (Japan). Hoechst 33,342 reagent was purchased from Beyotime (China). An Annexin-V FITC apoptosis detection kit (#556547) and BrdU cycle detection kit Part A (#559619) were purchased from BD Biosciences Pharmingen (United States). Antibodies against Bcl-2, Bax, Caspase 9/p35/p10, cyclin D1, and beta-actin were purchased from Proteintech (United States). Antibodies against cyclin A2, cyclin B1, p-Akt(S473), p-Akt(T308), pan-AKT, p-ERK (T202/Y204), and pan-ERK were purchased from Cell Signaling Technology (United States). Anti-CDK2 antibody was purchased from Abcam (United Kingdom). HSG was provided by Beijing Kangrentang Pharmaceutical Co., Ltd. The concentration of the prepared HSG mother solution was 0.1 g/mL. For the biological experiments, the 0.1 g/mL stock decoction could be subjected to 0.22-µm filter-sterilization and dilution as needed.