All isolates were spa typed based on the highly polymorphic region X of the protein A gene, which is composed of a variable number of 24-bp repeats [18 (link)]. The spa types were determined with the StaphType software version 2.2.1 (Ridom GmbH, Münster, Germany). spa-clonal complexes (spa-CC) were assigned using the based upon repeat pattern (BURP) algorithm as implemented in StaphType using preset parameters as recommended ("exclude spa types that are shorter than 5 repeats" and "spa types are clustered if costs are less or equal than 4") [18 (link)]. This algorithm clusters related spa types in spa-CC based on similarities of the repeat patterns in the polymorphic region X of the protein A gene. The spa-CCs are congruent with multilocus sequence typing (MLST) [18 (link)].
Staphtype software version
Manufactured by Ridom
Sourced in Germany
StaphType software is a molecular typing tool used for the identification and characterization of Staphylococcus aureus strains. It provides a standardized, reproducible, and portable method for the genetic typing of this clinically important bacterial species.
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Lab products found in correlation
2 protocols using staphtype software version
1
Spa Typing and Clonal Complex Analysis
2
Genotyping of Staphylococcus aureus
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Single-locus DNA sequencing of S. aureus protein A (spa) was carried out following standard methodology 14 and the obtained sequences were analysed by Ridom Staph-Type software version 1.5.21 (Ridom) for all MSSA-PEN S isolates. MLST (https://pubmlst.org/organisms/staphylococcusaureus) was performed for some selected isolates. The presumptive CCs associated with MLST of the isolates were assigned according to the spa type detected. 14
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