After the experiment (14 days) desirable tissues from glyphosate-treated and control plants were collected. Then tissues were washed in saline solution (0.75%), homogenized in 0.2 M pH 7.4 phosphate buffer (2 mL) and centrifuged (8000 rpm) at 4°C for 25 minutes. The supernatants were kept in Teflon tubes and stored at -80°C to perform the biochemical analysis of carbohydrate and total protein. Leaves were separated and put in acetone (80%, Sisco Research Laboratories) for the analysis of chlorophyll, i.e., chlorophyll a (Chlo-a), chlorophyll b (Chlo-b) and total chlorophyll (Chlo-t) [18 (link)].
Acetone
Manufactured by Sisco Research Laboratories
Sourced in India
Acetone is a clear, colorless liquid used as a solvent in various industrial and laboratory applications. It has a characteristic sweet, pungent odor. Acetone is commonly used as a solvent for numerous organic compounds, including paints, varnishes, lacquers, and adhesives.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Sisco Research Laboratories (3 mentions)
Ethanol, by Sisco Research Laboratories (3 mentions)
Chloroform, by Sisco Research Laboratories (3 mentions)
Bovine serum albumin (bsa), by HiMedia (2 mentions)
Dimethyl sulfoxide (dmso), by Sisco Research Laboratories (2 mentions)
Sodium hydroxide (naoh), by Sisco Research Laboratories (2 mentions)
Alamar blue, by HiMedia (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Glycine, by Sisco Research Laboratories (2 mentions)
Sodium acetate, by Sisco Research Laboratories (2 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by HiMedia (1 mentions)
Ferric chloride, by HiMedia (1 mentions)
Phenol, by Sisco Research Laboratories (1 mentions)
Hydrogen peroxide, by HiMedia (1 mentions)
Potassium hydroxide, by HiMedia (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Curcumin, by Merck Group (1 mentions)
Methylene blue, by Merck Group (1 mentions)
Dialysis membrane, by HiMedia (1 mentions)
15 protocols using acetone
1
Biochemical Analysis of Glyphosate-Treated Plants
2
Extraction and Characterization of Melanin
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Standard melanin (synthetic) and 5,5′-dithio-bis (2-nitrobenzoic acid) were purchased from Sigma-Aldrich, USA. Keratin powder, bovine serum albumin, sodium hydroxide, hydrogen peroxide, ferric chloride, sodium hypochlorite, and potassium hydroxide were obtained from Himedia, India. Solvents, such as ethanol, methanol, chloroform, acetone, benzene, and phenol were procured from Sisco Research Laboratory (SRL), India. All the chemicals and reagents used were of the highest purity and analytical grade. Black feathers of slaughtered domestic fowl (Gallusgallus domesticus) collected from central chicken market, Kolhapur, India were washed, dried, and stored for further studies. Whereas, goat (Capra hircus-Osmanabadi breed) skin was obtained from the local meat market in Kolhapur, India.
3
Curcumin-Loaded PLGA Nanoparticles
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Curcumin, Poly (lactic-co-glycolic acid) (PLGA), Poly-vinyl alcohol (PVA) and methyl thiazole tetrazolium (MTT) were purchased from Sigma Aldrich. Acetone and Dimethyl Sulfoxide (DMSO) was obtained from Sisco Research Laboratories Pvt. Ltd. (SRL).
4
Characterization of P. monodon Shells
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P. monodon (Fabricius, 1798) shells were collected from the Estuary of Southeast coast of Mandapam, Tamil Nadu, India. Sodium hydroxide, Acetone, Ethanol and Hydrochloric acid used were purchased from Sisco Research Laboratories Pvt. Ltd., Mumbai, India, and Dialysis membrane was purchased from HiMedia Laboratories, Mumbai, India. Methylene Blue, Bromophenol Blue and Coomassie Brilliant Blue were purchased from Sigma–Aldrich, USA.
5
Anticancer Compound Evaluation Protocol
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Malic acid, Diosgenin, Pluronic F68, 1, 12-Dodecanedioic acid (DDA), Pluronic 127 were purchased from Sigma Aldrich. Acetone was obtained from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Perchloric acid was purchased from Central Drug House (CDH) (Mumbai, India). For animal cell culture studies, Phosphate buffered saline, Alamar Blue and DAPI or 4′,6-diamidino-2-phenylindole, sodium bicarbonate were procured from HiMedia (Mumbai, India). Dulbecco’s modified Eagle’s medium (DMEM), Fetal Bovine Serum (FBS), Trypsin-EDTA (0.25%) were purchased from Gibco Life Technologies (Carlsbad, CA, USA). MilliQ water was used in the preparation of solutions during the study. A549, human adenocarcinoma cell lines were purchased from NCCS, Pune, India.
6
Synthesis and Characterization of Organic Compounds
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p-Phenylenediamine was purchased from TCI Chemicals (India) Pvt. Ltd. Xylene, toluene, chloroform, dichloromethane, 1,4-dioxane, acetone and silica gel were purchased from SISCO Research Laboratories Pvt. Ltd., India. Methanol and ethanol were purchased from Qualigens Pvt. Ltd. Benzene, 1-butanol, 2-propanol, DMSO, and DMF were obtained from Merck Life Science Pvt. Ltd. MWCO = 1 kDa dialysis membrane (HiMedia) was used as received. All commercially available reagent grade chemicals were used as received without further purification. Deionized (DI) water (resistivity ≈ 18 MΩ), determined using broadband dielectric measurements (Novocontrol broadband dielectric spectrometer in the frequency range of 10−2 to 107 Hz) was used for all the experiments reported.
7
Two-Dimensional Gel Electrophoresis Protocol
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Acrylamide, agarose, bovine serum albumin (BSA), bromophenol blue, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS), dithiothreitol (DTT), Histopaque (specific gravity 1.077), iodoacetamide (IAA), methylenebisAcrylamide, phenylmethane sulfonyl fluoride (PMSF), and Trizma were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Immobilized pH gradient strips, BioLyte 3/10, mineral, 2-DE cleanup kit, and 2-DE sodium dodecyl sulfate polyAcrylamide gel electrophoresis (SDS-PAGE) marker proteins (cat. no. 161-0320) were purchased from Bio-Rad (Hercules, CA, USA). Acetic acid, acetone, ammonium persulfate, ethanol, glycine, silver nitrate, sodium carbonate, sodium chloride, sodium dihydrogen phosphate, disodium hydrogen orthophosphate, sodium dodecyl sulfate (SDS), sodium thiosulfate, urea, and thiourea were purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Glycerol and formaldehyde were procured from Qualigen (Carlsbad, CA, USA). All other chemicals used were of analytical grade.
8
Superhydrophobic Coating Fabrication Protocol
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Polyvinylidene fluoride-co-hexafluoropropylene (PVDF-co-HFP, average Mw~400,000), 1H, 1H, 2H, 2H perfluorodecyltriethoxysilane (FAS, 97%), hexadecane (99%) was procured from Sigma-Aldrich, St. Louis, MO, USA. N, N dimethylformamide (DMF, >99.5%), acetone (>99.5%), isopropyl alcohol (IPA, >99.5%), sodium dodecyl sulphate (SDS, >99.0%) cetyltrimethylammonium bromide (CTAB, >99.0%), Tween-80 (≥98%) and alumina nanopowder—Alpha (20–30 nm, >99.9%) were obtained from Sisco Research Laboratories Pvt. Ltd., Mumbai, India. Analytical reagent grade solvents such as ethanol and methanol (>99.5%) and sodium chloride (NaCl, ≥99.0%), hydrochloric acid 35% (HCl, >99.5%) and sodium hydroxide (NaOH, 97%) were obtained from Merck, Mumbai India and used without further purification. Distilled water (<2 µS/cm) was used throughout the study.
9
Blending PLA and PBS for Sustainable Composites
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Poly(l -lactic acid) (PLA) (grade:
4032D, 98.6 ofd -lactic and 1.4 of l -lactic acid
unit) with number-average
molecular weight, Mn, ∼ 150 000
Da and polydispersity index, PDI, ∼ 1.3 was purchased from
NatureWorks. Poly(butylene succinate) (grade: 1001MD) with Mn ∼ 88 000 Da and PDI ∼
2.2 was supplied by Showa Highpolymer Singapore Pte. Ltd. (Japan).
Dicumyl peroxide (DCP, purity >99.5%) was purchased from Sigma
Aldrich (India) and used as a radical initiator. Whatmann filter paper
(grade: 1) was used to extract the functionalized chitosan (FCH). Acetone was supplied by SISCO Research Laboratories
(SRL chemicals, India). All of the chemicals have been consumed without
any further purification.
4032D, 98.6 of
unit) with number-average
molecular weight, Mn, ∼ 150 000
Da and polydispersity index, PDI, ∼ 1.3 was purchased from
NatureWorks. Poly(butylene succinate) (grade: 1001MD) with Mn ∼ 88 000 Da and PDI ∼
2.2 was supplied by Showa Highpolymer Singapore Pte. Ltd. (Japan).
Dicumyl peroxide (DCP, purity >99.5%) was purchased from Sigma
Aldrich (India) and used as a radical initiator. Whatmann filter paper
(grade: 1) was used to extract the functionalized chitosan (FCH). Acetone was supplied by SISCO Research Laboratories
(SRL chemicals, India). All of the chemicals have been consumed without
any further purification.
10
Analytical Assessment of Phytochemicals
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Analytical and HPLC grade chemicals were used in the study. Aluminium chloride, copper sulfate, ferric chloride, Folin-Ciocalteu phenol (FCP) reagent, potassium phosphate salts, sodium acetate, sodium hydroxide, sodium phosphate salts, sodium sulfate, and solvents (acetone, dimethyl sulfoxide (DMSO), methanol (MeOH), and petroleum ether) were purchased from Sisco Research Laboratories Pvt. Ltd., Mumbai, India; potassium persulphate (PPS) was purchased from the Central Drug House Pvt. Ltd., Delhi, India; concentrated hydrochloric acid (HCl), 2,2-diphenyl-2-picrylhydrazyl (DPPH), gallic acid (GA), L-ascorbic acid (A), potassium acetate, quercetin (Q), sodium carbonate, and 2,4,6-tripyridyl-s-triazine (TPTZ) were purchased from Himedia Co., Mumbai, India; 2,2’-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid or trolox (T), orlistat, and porcine PL Type-II (PPL) were purchased from Sigma Aldrich Co., St. Louis, MO, USA; betahistine hydrochloride tablets were from Abbott India Limited; sterile syringe filters (cellulose acetate, 0.2 μm pore size, 25 mm diameter) were purchased from Axiva Sichem Pvt. Ltd., Sonepat, Haryana, India.
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