BT549 and SKBR3 cells were obtained from the UCSF Cell Culture Facility.
BT20, BT474, HCC1428, HCC38, LY2, MCF7, MDAMB231, MDAMB453, T47D, SUM52PE, and
ZR75B cell lines were obtained from the American Type Culture Collection (ATCC).
Cell lines used for proteomic profiling and molecular analyses were
authenticated by STR analysis. Lines were grown according to published
protocols50 except for
SKBR3 which was cultured using RPMI media supplemented with 10% fetal
bovine serum (FBS) and 1% pen/strep. All cell lines tested negative for
mycoplasma contamination. Drugs used for cell culture experiments in this study
were purchased from Selleck Chemicals (GDC-0941, MK2206, PD0325901, Lapatinib,
MLN8237, and LEE011) and LC Laboratories (RAD001).
BT20, BT474, HCC1428, HCC38, LY2, MCF7, MDAMB231, MDAMB453, T47D, SUM52PE, and
ZR75B cell lines were obtained from the American Type Culture Collection (ATCC).
Cell lines used for proteomic profiling and molecular analyses were
authenticated by STR analysis. Lines were grown according to published
protocols50 except for
SKBR3 which was cultured using RPMI media supplemented with 10% fetal
bovine serum (FBS) and 1% pen/strep. All cell lines tested negative for
mycoplasma contamination. Drugs used for cell culture experiments in this study
were purchased from Selleck Chemicals (GDC-0941, MK2206, PD0325901, Lapatinib,
MLN8237, and LEE011) and LC Laboratories (RAD001).