Spme inlet liner

Manufactured by Merck Group

The SPME inlet liner is a component used in gas chromatography (GC) systems. Its core function is to facilitate the introduction of volatile and semi-volatile analytes into the GC instrument for separation and detection. The liner is designed to accommodate the solid phase microextraction (SPME) sampling technique, providing a controlled environment for the desorption of analytes from the SPME fiber.

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Lab products found in correlation

Slb 5ms column, by Merck Group (2 mentions) Agilent 5977b, by Agilent Technologies (2 mentions) Agilent 7820a, by Agilent Technologies (1 mentions) Agilent 7890a series gc, by Agilent Technologies (1 mentions) Hp 5ms, by Agilent Technologies (1 mentions) Msd chemstation software, by Agilent Technologies (1 mentions)

Spelling variants of this product

SPME liner (5 citations) SPME inlet (2 citations)

3 protocols using spme inlet liner

GC-MS Analysis of Volatile Compounds

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An Agilent 7820A gas chromatograph connected to
an Agilent 5977B mass selective detector (Agilent Technologies, Inc.,
Santa Clara, CA, USA) was used for all analyses. Separations were
performed on an SLB-5 ms column (30 m × 0.25 mm × 0.25 μm
df; Supelco). Helium carrier gas was used throughout this work, with
a constant flow rate of 1 mL min^–1. The system was
equipped with a SPME Merlin Microseal (Merlin Instrument Company,
Newark, DE, USA), and the inlet was maintained at a temperature of
250 °C. Splitless injection was used for all samples, and each
SPME fiber was desorbed for 2 min within a SPME inlet liner (Supelco).
The initial GC oven temperature was 40 °C for 5 min after which
the oven was temperature-programmed to increase at a rate of 10 °C
min^–1 to 270 °C. The MS was operated at a scan
rate of 3.94 s^–1, with a scan range of 35–400 m/z, ion source temperature 230 °C
and ionizing energy of 70 eV.

Finnegan M., Fitzgerald S., Duroux R., Attia J., Markey E., O’Connor D, & Morrin A. (2024). Predicting Chronological Age via the Skin Volatile Profile. Journal of the American Society for Mass Spectrometry, 35(3), 421-432.

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GC-MS Analysis of Volatile Compounds

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Following HS sampling, SPME fibers were injected into an Agilent 7890A series GC connected to an Agilent 5977B mass selective detector (Agilent Technologies, Inc., Santa Clara, CA, USA) for analysis. Separations were performed on an SLB-5ms column (30 m × 0.25 mm, d

_{f}

0.25

μ

m; Supelco, Bellefonte, PN, USA). Helium was used as the carrier gas at 1 mL/min flow rate. An SPME Merlin Microseal (Merlin Instrument Company, Newark, DE, USA) was installed, and the GC injection port was maintained at a temperature of 260 °C. Splitless injection was used for all samples, and the SPME fiber was desorbed for 4 min within an SPME inlet liner (Supelco). The oven temperature program was isothermal for the first 4 min at 35 °C, then raised to 120 °C at a rate of 5 °C/min, holding for 2 min, then raised again to 270 °C at a rate of 10 °C/min and held for 2 min with a total run time of approx. 40 min. The transfer line to the MS was maintained at 250 °C. The MS was operated with a scan range of 33–330 m/z, ion source temperature of 230 °C, and an ionizing energy of 70 eV.

Finnegan M., Thach C.L., Khaki S., Markey E., O’Connor D.J., Smeaton A.F, & Morrin A. (2023). Characterization of Volatile and Particulate Emissions from Desktop 3D Printers. Sensors (Basel, Switzerland), 23(24), 9660.

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Agilent GC-MS Analysis of Apple Volatiles

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Apple volatiles were analysed on an Agilent 6890N gas chromatograph (GC) equipped with a single quadrupole mass spectrometer detector 5973N (MS). Separation was achieved on a HP-5-MS (30 m x 0.25 mm i.d., 0.1 m film thickness, Agilent, Folsom, CA, USA) capillary column which was exposed to the following oven temperature program: 45˚C held for 6 min, raised up to 130˚C at 10˚C/min, then to 180˚C at 3˚C/min, finally to 300˚C at 20˚C/min held for 3 min. The GC injector port was equipped with a SPME inlet liner (0.75 mm i.d, Supelco) operating in splitless mode. Carrier gas was helium with a flow rate of 1.0 mL/min Injector and transfer line temperatures were set up to 250°C and to 280°C, respectively. The acquisition of detector was in the 29-400 m/z range. Chromatograms were analysed using the MSD ChemStation software (Agilent, Version G1701DA D.01.00) and the NIST Mass Spectral Search Program for the NIST/EPA/NIH Mass Spectral Library v. 2.0. Mass spectra (MS) of the peaks were studied using the following libraries: ADAMS, NIST 17 and WILEY275. In addition, the temperature-programmed retention indices. The relative abundance of components, expressed in percentages, were extrapolated from the peak areas without using correction factors.

Nkuimi Wandjou J.G., Sut S., Giuliani C., Fico G., Papa F., Ferraro S., Caprioli G., Maggi F, & Dall'Acqua S. (2019). Characterization of nutrients, polyphenols and volatile components of the ancient apple cultivar 'Mela Rosa Dei Monti Sibillini' from Marche region, central Italy. International journal of food sciences and nutrition, 70(7).

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