HEK-293T cells were transiently transfected using Lipofectamine 2000 (Life Technologies). Cells were lysed in 0.5% TNTE. After pre-clearing with protein G-Sepharose, lysates were incubated with anti-Flag M2 antibody (Sigma) or anti-Hemagglutinin (HA)-antibody (Roche) bound to Protein-G Dynabeads (Life TechnologiesTM) for 2 hours at 4°C. Immunoprecipitates were washed 5 times with 0.1% TNTE, subjected to SDS-PAGE, transferred onto nitrocellulose and immunoblotted with the anti-Hemagglutinin (HA)-antibody (Roche) or anti-Flag M2 antibody (Sigma). Detection was achieved using horseradish peroxidase-conjugated rabbit anti-rat (Sigma) or sheep anti-mouse secondary antibodies (GE Healthcare) and chemiluminescence. ImageJ was used for quantification of band intensities.
Anti hemagglutinin ha antibody
Manufactured by Roche
Sourced in Switzerland
The Anti-Hemagglutinin (HA)-antibody is a laboratory product used for the detection and analysis of the hemagglutinin protein, which is a key component of the influenza virus. This antibody can be utilized in various immunoassay techniques to identify and quantify the presence of the hemagglutinin protein in biological samples.
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Lab products found in correlation
Sheep anti mouse secondary antibody, by GE Healthcare (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Anti flag m2 antibody, by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Triciribine, by Merck Group (1 mentions)
Wortmannin, by Merck Group (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
Pifithrin α, by Merck Group (1 mentions)
P21cip1 waf1, by Santa Cruz Biotechnology (1 mentions)
Rhodamine conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Nutlin 3, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Cycloheximide (chx), by Merck Group (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti hemagglutinin ha antibody
1
Immunoprecipitation Assay for Protein-Protein Interactions
2
Evaluating Protein Interactions in Cell Signaling
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Antibodies against CK2α, p53, p21Cip1/WAF1, and β-actin were obtained from Santa Cruz Biotechnology (USA). An antibody against phospho-p53 serine 392 (S392) was purchased from Cell Signaling Technology (USA), and antibodies against HP1γ, SUV39h1, H3K9me3, histone H3, and MDM2 were from Abcam (UK). Both 4′,6-diamidino-2-phenylindole (DAPI) and rhodamine-conjugated goat anti-rabbit IgG were purchased from Invitrogen (USA). The anti-hemagglutinin (HA) antibody was obtained from Roche (Switzerland) and TrueBlot reagent was from Rockland (USA). Pifithrin-α, nutlin-3, cycloheximide, N-Acetyl-L-cysteine (NAC), wortmannin, triciribine, and rapamycin were obtained from Sigma Chemical (USA).
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