Espion 100

Full-field electroretinography (ERG) recordings were conducted as described previously (Xu et al., 2012 (link)). Briefly, after overnight dark adaptation, mice were anesthetized by intraperitoneal injection of 85 mg/kg ketamine and 14 mg/kg xylazine. ERGs were recorded using the Espion Visual Electrophysiology System (Diagnosys) with the ColorDome Advanced Performance Ganzfeld Dome system (Diagnosys). Potentials were recorded using a gold-wire electrode to contact the corneal surface through a layer of 2.5% hypromellose (Gonak, Akorn Pharmaceuticals). For the assessment of scotopic responses, a stimulus intensity of 1.89 log cd · s m⁻² was presented to dark-adapted dilated mouse eyes. To evaluate photopic responses, mice were adapted to a 1.48 log cd · s m⁻² light for 7 min, and then a light intensity of 1.89 log cd · s m⁻² was given. Responses were differentially amplified, averaged, and analyzed using Espion 100 software (Diagnosys).

Full-field electroretinography (ERG) recordings were conducted as described previously (Xu et al., 2011 (link)). Briefly, after overnight dark adaptation, mice were anesthetized by intraperitoneal injection of 85 mg/kg ketamine and 14 mg/kg xylazine. ERGs were recorded using an Espion visual electrophysiology system with a Ganzfeld ColorDome system (Diagnosys). Potentials were recorded using a gold-wire electrode to contact the corneal surface through a layer of 2.5% hypromellose (Gonak, Akorn). For assessment of scotopic responses, a stimulus intensity of 2.20 log cd·s m⁻² was presented to dark-adapted dilated mouse eyes. To evaluate photopic responses, mice were adapted to a 1.48 log cd·s m⁻² light for 5 min, and then a light intensity of 1.89 log cd·s m⁻² was given. Responses were differentially amplified, averaged, and analyzed using Espion 100 software (Diagnosys).