Mouse monoclonal anti trf1

Hydrogen peroxide (H₂O₂) was obtained from Junsei (Tokyo, Japan). FK866 was purchased from Adipogen (Switzerland). The antibodies used in the present study were as follows—rabbit anti-p21 (Santa Cruz Biotech, Dallas, TX, USA), mouse monoclonal anti-p53 (Calbiochem, San Diego, CA, USA), mouse monoclonal anti-β-Actin (Abcam, Cambridge, MA, USA), mouse monoclonal anti-TRF1 (Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-phospho (Ser139)-histone H2AX (γH2AX) (Cell Signaling Technology, Danvers, MA, USA), mouse monoclonal anti-NF-κB p65 (Santa Cruz Biotech, Dallas, TX, USA), horseradish peroxidase-conjugated goat anti-rabbit, and anti-mouse IgG (Thermo Fisher Scientific, Waltham, MA, USA), Alexa Fluor^® 488-conjugated goat anti-mouse IgG, and Alexa Fluor^® 594-conjugated goat anti-rabbit IgG (Invitrogen, Camarillo, CA, USA).

H₂O₂ was obtained from Junsei (Tokyo, Japan). Visfatin and FK866 were purchased from Adipogen (San Diego, CA, USA). Primary antibodies used in this study were as follows: rabbit anti-Visfatin (Adipogen, San Diego, CA, USA), rabbit anti-p21 (Santa Cruz Biotech, Dallas, TX, USA), mouse monoclonal anti-p53 (Calbiochem, San Diego, CA, USA), rabbit anti-α-Tubulin (Bioworld, Minneapolis, MN, USA), mouse monoclonal anti-β-Actin (Abcam, Cambridge, MA, USA), mouse monoclonal anti-c-Myc (Santa Cruz Biotech, Dallas, TX, USA), mouse monoclonal anti-TRF-1 (Santa Cruz Biotech, Dallas, TX, USA), rabbit anti-phospho (Ser139)-histone H2AX (γH2AX) (Cell Signaling Technology, Danvers, MA, USA), and mouse monoclonal anti-NF-κB p65 (Santa Cruz Biotech, Dallas, TX, USA). Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse IgG were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Alexa Fluor^® 488-conjugated goat anti-mouse IgG, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG, Alexa Fluor^® 594-conjugated goat anti-mouse IgG, and Alexa Fluor^® 594-conjugated goat anti-rabbit IgG were purchased from Invitrogen (Camarillo, CA, USA).