Snx1 clone 51 snx1

Manufactured by BD

Sourced in United States

SNX1 (clone 51/SNX1) is a laboratory equipment product manufactured by BD. It is a reagent that can be used for the detection of the SNX1 protein in various experimental settings. The core function of this product is to provide a tool for researchers to study the expression and localization of the SNX1 protein in biological samples.

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Lab products found in correlation

Snx2 clone 13 snx2, by BD (2 mentions) Itga5, by BD (2 mentions) Vps35, by Abcam (2 mentions) β actin, by Merck Group (2 mentions) Tgn46, by Bio-Rad (1 mentions) Golgin97 clone cdf4, by Thermo Fisher Scientific (1 mentions) Galnt2, by Abcam (1 mentions) Calnexin, by Abcam (1 mentions) Tom20, by BD (1 mentions)

2 protocols using snx1 clone 51 snx1

Antibody Characterization for Cell Biology

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Antibodies used in this study were as follows: GFP (clones 7.1 and 13.1; 11814460001; Roche) (1:2,000 for Western blot (WB), 1:400 for immunofluorescence (IF)), SNX1 (clone 51/SNX1; 611482; BD) (1:1,000 for WB, 1:200 for IF), SNX2 (clone 13/SNX2; 5345661; BD) (1:1,000 for WB, 1:200 for IF), SNX6 (clone d-5,365965; Santa Cruz Biotechnology, Inc.) (1:1,000 for WB, 1:200 for IF), SNX5 (clone EPR14358; ab180520; Abcam) (WB 1:500), β-actin (A1978; Sigma-Aldrich) (1:2,000 for WB), ITGA5 (610633, BD) (1:1,000 for WB); VPS35 (97545; Abcam) (IF 1:200), GFP (GTX20290; GeneTex) (WB 1:2,000), EEA1 (C45B10; Cell Signaling Technologies) (IF 1:200), LAMP1 (clone H4A3, AB2296838; DSHB) (IF 1:200) Cathepsin D (21327-1-AP; Proteintech) (WB 1:1,000), Calnexin (ab22595; Abcam) (WB 1:1,000), TGN46 (AHP500G, Bio-Rad) (IF 1:400), Golgin-97 (clone CDF4; A-21270; Thermo Fischer) (IF 1:200), HA tag (66006-1-Ig, Proteintech) (WB 1:1,000), NCAD (clone 13A0; 14215; Cell Signaling) (WB 1:1,000), Tom20 (clone 29; 612278; BD Biosciences), GALNT2 (ab102650; Abcam) (WB 1:1,000), NRP1 (clone EPR3113; ab81321; Abcam) (WB 1:1,000, IF 1:200), NRP1 b1b2 domain (23 (link), 24 (link)) (clone 3E7.1) (IF 1:200), Tubulin (ab6046; Abcam) (WB 1:2,000), SARS-CoV-2 spike (40592-T62, Sino Biologicals) (WB 1:1,000) SARS Nucleocapsid (200-401-A50, Rockland) (IF 1:2,000).

Simonetti B., Daly J.L., Simón-Gracia L., Klein K., Weeratunga S., Antón-Plágaro C., Tobi A., Hodgson L., Lewis P.A., Heesom K.J., Shoemark D.K., Davidson A.D., Collins B.M., Teesalu T., Yamauchi Y, & Cullen P.J. (2022). ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1. Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2201980119.

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Antibody Characterization for Trafficking Studies

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Antibodies used in this study were mouse monoclonal antibodies GFP (clones 7.1 and 13.1; 11814460001; Roche, Germany) (1:2,000 for WB), LAMP1 (clone H4A3, AB2296838; DSHB, USA) (IF 1:200), SNX1 (clone 51/SNX1; 611482; BD, USA) (1:1,000 for WB, 1:200 for IF), SNX2 (clone 13/SNX2; 5345661; BD) (1:1,000 for WB, 1:200 for IF), SNX6 (clone d-5, 365965; Santa Cruz Biotechnology, USA) (1:1,000 for WB, 1:200 for IF), SNX27 (ab77799, Abcam, UK) (1:1,000 for WB, 1:200 for IF), β-actin (A1978; Sigma-Aldrich, USA) (1:2,000 for WB), ITGA5 (610633, BD) (1:1,000 for WB), VPS29 (clone d-1, 398874; Santa Cruz Biotechnology) (1:200 for WB); rabbit monoclonal antibodies: EEA1 (C45B10; Cell Signaling Technology, USA) (IF 1:200), VPS35 (EPR11501(B); 157220; Abcam) (1:1,000 for WB), GLUT1 (ab115730, Abcam) (1:1,000 for WB, 1:200 for IF); rabbit polyclonal antibodies: GFP (GTX20290; GeneTex, USA) (WB 1:2,000), VPS26A (23892; Abcam) (1:1,000 for WB), VPS35 (97545; Abcam) (1:1,000 for WB, 1:200 for IF), VPS29 (98929; Abcam) (1:100 for WB); and goat polyclonal antibodies to VPS35 (10099; Abcam) (1:200 for IF).

Simonetti B., Guo Q., Giménez-Andrés M., Chen K.E., Moody E.R., Evans A.J., Chandra M., Danson C.M., Williams T.A., Collins B.M, & Cullen P.J. (2022). SNX27–Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling. PLoS Biology, 20(4), e3001601.

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