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Horseradish peroxidase hrp linked antibodies

Manufactured by GE Healthcare
Sourced in United Kingdom

Horseradish peroxidase (HRP)-linked antibodies are protein molecules that have been conjugated with the enzyme horseradish peroxidase. This enzyme-linked antibody is commonly used in various immunoassay techniques, such as Western blotting and enzyme-linked immunosorbent assay (ELISA), to detect and quantify target proteins or other biomolecules.

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2 protocols using horseradish peroxidase hrp linked antibodies

1

Antibody Panel for Immunoblot Analysis

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Anti-FLAG mouse mAb was purchased from Sigma (St. Louis, MO), and anti-c-myc mAb was from Roche Applied Science (Penzberg, Germany); anti-EphA2 (N-terminal) mAb was from R&D Systems (Minneapolis, MN); anti-EphA2 (C-terminal) pAb) and anti-phospho-Erk1/2 (p-T202/p-Y204) mAb, anti-Erk pAb, anti-EphA2 mAb were from Santa Cruz Biotechnology (Dallas, TX); anti-Akt mAb and phospho-Akt (p-S473) mAb were from Cell Signaling Technology (Danvers, MA); anti-phospho-EphA2 (p-S897) pAb and anti-phospho-EphA2 (p-Y594) pAb were from Cell Applications (San Diego, CA); anti-phospho-Y mAb (4G10) and anti-actin mAb were from Millipore (Billerica, MA). Horseradish peroxidase (HRP)-linked antibodies were bought from GE Healthcare (Little Chalfont, UK). The hydroxamate synthetic matrix metalloproteinase inhibitor, BB94, was provided by Dr. Peter D. Brown (British Biotech Pharmaceuticals Ltd.; Oxford, UK).
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2

Antibody Detection and Characterization

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Primary antibodies were used that recognise N-cadherin (BD transduction); α-catenin (Sigma C2081); β-catenin (BD transductions 610920); p120-catenin (BD Transduction 61034); ERK1/2 (Sigma M5670), mCherry (Abcam ab183628; western blotting); mCherry (Life Technologies M11217; Immunoprecipitation), AKT 1/2/3 (Santa Cruz), Slit2 (Abcam ab134166; western blotting), Slit2 (Thermo Fisher Scientific PA531133; immunofluorescence), Slit3 (Sigma SAB2104337; immunofluorescence) Slit3 (R&D Systems AF3629; western blotting), Myc (Merck Millipore 05-724). Alexa Fluor secondary antibodies were obtained from Invitrogen. Horseradish peroxidase (HRP)-linked antibodies were obtained from GE Healthcare.
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