Anti tyrosine hydrolase polyclonal rabbit antibody

Rotenone and TA of the highest purity were bought from Sigma Aldrich, Missouri, USA. The RIPA buffer and antibodies for glial fibrillary acidic protein (GFAP), COX-2, and iNOS were purchased from Sigma Aldrich, (St. Louis, MO, USA). The protease and phosphatase inhibitor combination were obtained from Thermo Fisher Scientific (Waltham, MA, USA). A polyclonal rabbit anti-tyrosine hydrolase antibody was obtained from Merck, Darmstadt, Germany. The antibodies, LC3, p62, mTOR, phosphor mTOR, and p70s6, were purchased from Cell Signaling Technology, (Danvers, MA, USA). The apoptotic polyclonal markers, Bax and Bcl-2, were purchased from (Cambridge, Abcam, MA, USA), and the monoclonal mouse anti-synuclein antibody was obtained from BD Biosciences, (San Jose, CA, USA). Anti-Iba-1 antibody was obtained from Wako Chemicals, Richmond, VA, USA. The fluorescent secondary antibody, Alexa Flour 488, was purchased from Thermo Fischer Scientific, (Waltham, MA, USA). The biotinylated secondary goat anti-rabbit antibody was obtained from Jackson immune research laboratory, (Baltimore Pike, West Grove, PA, USA). The biochemical assays were performed using commercially available kits. The additional compounds utilized in these tests were all purchased from regional vendors and were of analytical-grade quality.

Monoclonal mouse anti-α-synuclein was purchased from BD biosciences, San Jose, CA. Polyclonal rabbit anti-cyclooxygenase-2 (Cox-2), anti-inducible nitric oxide synthase (iNOS) and anti-glial fibrillary acidic protein (GFAP) were procured from Sigma-Aldrich, St. Louis, MO, USA. Anti-ionized calcium-binding adaptor molecule-1 (Iba-1) was purchased from Wako chemicals, Richmond, USA. Polyclonal rabbit anti-tyrosine hydrolase antibody was obtained from Merck, Germany. Antibodies against MMP-2 was purchased from Abcam, Cambridge, MA, USA. Alexa Flour 488 conjugated goat anti-rabbit secondary antibodies were purchased from Thermo Fischer Scientific, Waltham, MA, USA. Biotinylated secondary goat anti-rabbit antibody was purchased from Jackson Immunoresearch, West grove, USA. Lycopodium extract, Rotenone and other analytical grade reagents were procured from Sigma Aldrich, St. Louis, MO, USA. The biochemical assays were performed using commercially-available kits.