Magnetospirillum gryphiswaldense (MSR-1) was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Germany). MSR-1 was grown in a 5-L Biostat B (Sartorius Stedim UK Ltd., Surrey, UK) bioreactor following a pH-stat strategy described elsewhere (Fernández-Castané et al., 2018 (link)). Briefly, MSR-1 cells were grown at 30°C, under microaerobic conditions (pO2 < 1%.), in flask standard medium (FSM) without iron citrate at a pH of 7. The pH was maintained by automated addition of an acidic feeding solution. Once the culture reached stationary phase, MSR-1 cells were harvested by centrifugation (4,000 rpm, 20 min, 4°C) in a Heraeus Multifuge X1R centrifuge (Thermo Scientific, Massachusetts, United States). The supernatant was removed and the pellet was stored at −80°C until further use.
Multifuge x1r centrifuge
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Multifuge X1R centrifuge is a benchtop device designed for general laboratory applications. It features a fixed-angle rotor to separate samples based on density and particle size through centrifugal force. The Multifuge X1R has a maximum speed of 14,000 rpm and a maximum relative centrifugal force of 20,800 x g.
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Lab products found in correlation
Msr 1, by Leibniz Institute DSMZ (2 mentions)
5 l biostat b, by Sartorius (1 mentions)
Msr 1, by Thermo Fisher Scientific (1 mentions)
Nicolet is5 spectrometer, by Thermo Fisher Scientific (1 mentions)
Smart apex, by Bruker (1 mentions)
Jem 2100 transmission electron microscope, by JEOL (1 mentions)
8453 diode array spectrophotometer, by Agilent Technologies (1 mentions)
Tecnai f30h 7650 microscope, by Thermo Fisher Scientific (1 mentions)
Miliq h2o system, by Merck Group (1 mentions)
3 protocols using multifuge x1r centrifuge
1
Cultivation of Magnetospirillum gryphiswaldense
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Magnetospirillum gryphiswaldense (MSR-1) was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Germany). MSR-1 was grown in a 5-L Biostat B (Sartorius Stedim UK Ltd., Surrey, UK) bioreactor following a pH-stat strategy described elsewhere (Fernández-Castané et al., 2018 (link)). Briefly, MSR-1 cells were grown at 30°C, under microaerobic conditions (pO2 < 1%.), in flask standard medium (FSM) without iron citrate at a pH of 7. The pH was maintained by automated addition of an acidic feeding solution. Once the culture reached stationary phase, MSR-1 cells were harvested by centrifugation (4,000 rpm, 20 min, 4°C) in a Heraeus Multifuge X1R centrifuge (Thermo Scientific, Massachusetts, United States). The supernatant was removed and the pellet was stored at −80°C until further use.
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Magnetospirillum gryphiswaldense (MSR-1) was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ, Germany). MSR-1 was grown in a 5-L Biostat B (Sartorius Stedim UK Ltd., Surrey, UK) bioreactor following a pH-stat strategy described elsewhere (Fernández-Castané et al., 2018 (link)). Briefly, MSR-1 cells were grown at 30°C, under microaerobic conditions (pO2 < 1%.), in flask standard medium (FSM) without iron citrate at a pH of 7. The pH was maintained by automated addition of an acidic feeding solution. Once the culture reached stationary phase, MSR-1 cells were harvested by centrifugation (4,000 rpm, 20 min, 4°C) in a Heraeus Multifuge X1R centrifuge (Thermo Scientific, Massachusetts, United States). The supernatant was removed and the pellet was stored at −80°C until further use.
2
Comprehensive Characterization of CONs
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The morphology of the solvent was observed by scanning electron microscopy (SEM) on a SWPRA™ 55 scanning electron microscope manufactured by Carl Zeiss Micro Imaging Co., Ltd. (Jena, Germany). Transmission electron microscopy (TEM) was conducted on a JEM-2100 transmission electron microscope manufactured by JEOL Co., Ltd. (Tokyo, Japan). Thermogravimetric analysis (TGA) of the CONs was performed on an STA 449F3 Simultaneous Thermal Analyzer (Netsch Co., Ltd., Selb, Germany) between 60 and 800 °C under an N2 flow rate of 10 mL/min. Powder X-ray diffraction (PXRD) data were recorded using a Bruker Smart Apex charged coupled device-based diffractometer (Bruker Co., Ltd., Karlsruhe, Germany). The N2 adsorption-desorption isotherms (77 K) of the CONs were measured using a porosimeter (SAP 2460; Micromeritics Instruments Corporation, Norcross, GA, USA). Fourier transform-infrared (FTIR) spectroscopy was performed using a Nicolet iS5 Spectrometer obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Centrifugation was operated with a Multifuge X1R centrifuge obtained from Thermo Fisher Scientific Inc.
3
Characterization of Gold Nanoparticles
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A Foxy-RTM fluorescence O2 sensor from Ocean Optics (EW Duive, The Netherlands, https://oceanoptics.com/) consisting of a QE65000 CCD-array spectrometer coupled to an oxygen FOXY-R (by A QBIF600-UV/Vis optical fiber), and a LS-450 pulsed blue LED light as radiation source, was used to measure dissolved oxygen. A Tecnai F30H-7650 microscope (scanning and transmission mode, STEM) (FEI, The Netherlands, https:// www.fei.com) was used for characterization of the gold nanoparticles. UV-vis molecular absorption measurements were performed on a Specord 210 Plus spectrophotometer and an Agilent 8453 diode array spectrophotometer (One cm cuvettes were used in all cases). A Thermo Scientific Multifuge X1R centrifuge was used for separation by centrifugation. The Millipore MiliQ H2O system was used for water purification.
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