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Nahco3

Manufactured by PanEco

NaHCO3 is a chemical compound that is commonly used as a laboratory reagent. It is a white crystalline solid that has the chemical formula NaHCO3, which represents sodium bicarbonate. NaHCO3 is a versatile compound that can be used in various laboratory applications.

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3 protocols using nahco3

1

Cell Culture of TLR4 and NOD2 Reporters

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RAW-Blue, HEK-Blue-hTLR4, HEK-Blue-hNOD2, and control HEK-Blue-Null2 cells were obtained from Invivogen (San Diego, CA, USA) and maintained in Dulbecco‘s Modified Eagle’s medium (DMEM) medium (GE Healthcare, Chicago, IL, USA) supplemented with 10% fetal calf serum (Thermo Scientific, Waltham, MA, USA), 50 U/mL penicillin, 50 μg/mL streptomycin, 2 mM glutamine, and 0.1 M NaHCO3 (all PanEco, Moscow, Russia) at 37 °C with 5% CO2.
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2

Cell Culture for NF-κB/AP-1 Assay

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THP-1 cells, THP1-XBlue-CD14 cells as well as control cells HEK-Blue-null1 and HEK-Blue-null2 with NF-κB/AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter (all InvivoGen, USA) were cultured in RPMI and DMEM, respectively (PAA, USA). Media were supplemented with 10% fetal calf serum (Thermo Scientific, USA), 50 U/mL penicillin, 50 μg/mL streptomycin, 2 mM glutamine, 0.1 M NaHCO3 (all PanEco, Russia) at 37°C with 5% CO2.
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3

Luciferase Assay for NF-κB, NFAT, and AP-1 Activation

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Reporter cells (RAW-NF-κB-Luc, RAW-NFAT-Luc, and RAW-AP-1-Luc) were cultured in DMEM medium (GE Healthcare, USA) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, USA), 50 U/mL penicillin, 50 μg/mL streptomycin, 2 mM glutamine, and 0.1 M NaHCO3 (all PanEco, Russia) at 37°C with 5% CO2. We used the protocol described in our previous work [16 (link)]; namely, experiment cells were seeded at 1 × 105 cells/well in 96-well plates (100 μL/well). The next day, C-type lectin receptor agonists were added to the wells to a final concentration of 20 μg/mL, 4 μg/mL, and 1 μg/mL. Eight hours later, to detect luciferase activity, 100 μL of Bright-Glo Luciferase Assay Buffer containing luciferin substrate (Promega, USA) was added to each well. Luminescence was measured in relative units using a Synergy H4 Hybrid Reader (BioTek, Germany).
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